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Related Concept Videos

Chromosome Replication02:31

Chromosome Replication

Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin of...
The Replisome03:01

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DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...

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Related Experiment Video

Updated: May 13, 2026

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
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Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

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Eukaryotic replisome components cooperate to process histones during chromosome replication.

Magdalena Foltman1, Cecile Evrin, Giacomo De Piccoli

  • 1Paterson Institute for Cancer Research, University of Manchester, Wilmslow Road, Manchester M20 4BX, UK.

Cell Reports
|March 19, 2013
PubMed
Summary

Histones displaced during DNA replication are re-bound by the Mcm2 helicase and FACT complex. This interaction preserves chromatin structure and supports cell growth, highlighting conserved chaperone mechanisms.

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Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
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Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique
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Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique

Published on: January 14, 2016

Area of Science:

  • * Molecular Biology
  • * Epigenetics
  • * Chromatin Biology

Background:

  • * Eukaryotic DNA replication requires parental histone redeposition to maintain chromatin structure.
  • * Histone chaperones are essential for managing histones during DNA replication and transcription.
  • * The Mcm2-7 helicase complex is central to DNA replication initiation and elongation.

Purpose of the Study:

  • * To identify replication machinery components involved in histone binding during DNA replication.
  • * To elucidate the role of the FACT complex in conjunction with the Mcm2 helicase in histone management.
  • * To investigate the functional significance of Mcm2-histone interactions for chromatin integrity and cell viability.

Main Methods:

  • * Systematic screening of yeast cell extracts for histone-binding replisome components.
  • * Co-immunoprecipitation assays to detect protein-protein interactions.
  • * Genetic analyses to assess the impact of Mcm2 mutations on chromatin stability and cell growth.

Main Results:

  • * Mcm2 helicase subunit binds histones cooperatively with the FACT complex.
  • * FACT is incorporated into the replisome progression complex independently of histone binding.
  • * Mcm2's N-terminal tail motif binds histones, preserving subtelomeric chromatin and minichromosome stability.
  • * This interaction supports growth in specific genetic backgrounds (e.g., absence of Ctf18-RFC).

Conclusions:

  • * Eukaryotic replication and transcription machineries utilize analogous multi-chaperone assemblies for chromatin preservation.
  • * The Mcm2-FACT interaction is crucial for efficient histone redeposition and maintaining genome stability.
  • * Conserved mechanisms involving histone chaperones are vital for preserving chromatin integrity during DNA replication.