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Related Experiment Video

Updated: May 13, 2026

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier (MSC) for Lung Cancer Screening
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Published on: October 26, 2017

Feasibility of circulating miRNA microarray analysis from archival plasma samples.

Maurizio Callari1, Paola Tiberio, Loris De Cecco

  • 1Unit of Biomarkers, Department of Experimental Oncology and Molecular Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy.

Analytical Biochemistry
|March 19, 2013
PubMed
Summary

Circulating microRNAs show promise as disease biomarkers. This study validates microarray methods for reproducible detection in archival plasma, addressing technical challenges for reliable biomarker measurement.

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Last Updated: May 13, 2026

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Area of Science:

  • Biomarker Discovery
  • Molecular Diagnostics
  • Genomics

Background:

  • MicroRNAs are implicated in various diseases and are stable in body fluids, making them potential noninvasive biomarkers.
  • Accurate measurement of circulating microRNAs (miRNAs) is crucial but challenged by technical variables.
  • Archival plasma samples are valuable but require validation for miRNA analysis.

Purpose of the Study:

  • To assess the reliability of microarray-based miRNA profiling for clinical applications.
  • To evaluate the feasibility of using long-term stored, limited archival plasma samples.
  • To identify and address technical factors influencing circulating miRNA measurements.

Main Methods:

  • Utilized a microarray-based platform for high-throughput miRNA profiling.
  • Assessed intra- and inter-array reproducibility of the miRNA profiling method.
  • Evaluated the correlation between different batches of miRNA measurements.
  • Investigated the impact of sample storage duration and chip expiration on signal background.

Main Results:

  • Demonstrated adequate intra- and inter-array reproducibility for miRNA profiling.
  • Confirmed the feasibility of analyzing miRNAs from archival plasma samples, even with limited quantities.
  • Observed good correlation across different experimental batches.
  • Identified a time-dependent increase in background signals as the microarray chip approached its expiration date.

Conclusions:

  • Microarray-based profiling is a reproducible method for detecting circulating miRNAs.
  • Archival plasma samples can be reliably used for miRNA biomarker studies.
  • Understanding and mitigating technical variables, such as chip expiration, is essential for accurate miRNA biomarker quantification.