Related Experiment Video
Updated: May 13, 2026

A Seamless Cloning Approach for Porcine Reproductive and Respiratory Syndrome Virus Expression Vector Construction
Published on: May 17, 2024
Cellular pathogenesis of porcine circovirus type 2 infection
Cheryl M T Dvorak1, Sumathy Puvanendiran, Michael P Murtaugh
1Department of Veterinary and Biomedical Sciences, University of Minnesota, 1971 Commonwealth Ave, St. Paul, MN 55108, USA. dvora013@umn.edu
Abstract:
Porcine circovirus associated disease (PCVAD) and the associated histological lesions are thought to appear due to an increase in the amount of porcine circovirus type 2 (PCV2) present in an infected animal. However, examination of the cellular and molecular pathogenesis of PCVAD is complicated by the lack of a consistent cell culture model that replicates the animal phenotypes of persistent, asymptomatic infection, and acute, pathological disease typified by lymphocyte depletion. The porcine fetal retina cell line, VR1BL, shows a high permissiveness to PCV2 infection, 40 times higher than the alternative PK15 culture model, allowing for high titer viral production, with PCV2b growth higher than PCV2a growth. Cytopathic effect due to apoptosis is observed after challenge with high amounts of PCV2, but at low levels, infection is maintained in passaged cells. Thus, VR1BL cells may be used as a model system to examine both acute viral pathogenesis and cellular innate defense, as well as persistent PCV2 infection.
More Related Videos
Related Concept Videos
Acute Pancreatitis II: Pathophysiology
Pneumonia II: Pathophysiology
Leaky Scanning
Chronic Pancreatitis II: Pathophysiology
Cystic Fibrosis: Pathogenesis
CF is primarily caused by a genetic mutation in a chromosome 7 gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. The most common gene mutation leading to CF is the ΔF508 mutation, but...
Encephalitis ll: Pathophysiology

