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Related Experiment Videos

Cellular gene expression for calbindin-D28k in mouse kidney.

W B Rhoten1, S Christakos

  • 1Department of Anatomy, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark 07103-2757.

The Anatomical Record
|June 1, 1990
PubMed
Summary

Calbindin-D28k gene expression is highly concentrated in the distal tubules of the mouse kidney. This vitamin D-dependent calcium-binding protein is primarily found in these specific nephron cells.

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Area of Science:

  • Nephrology
  • Molecular Biology
  • Cell Biology

Background:

  • Calbindin-D28k is a vitamin D-dependent calcium-binding protein.
  • Its precise localization within the nephron is crucial for understanding kidney function.

Purpose of the Study:

  • To investigate the gene expression patterns of calbindin-D28k within the murine nephron.
  • To determine the specific cellular locations of calbindin-D28k mRNA.

Main Methods:

  • In situ hybridization (hybridization cytochemistry) on murine nephron tissue sections.
  • Use of radiolabeled single-stranded RNA complementary to calbindin-D28k mRNA as a probe.
  • Autoradiography and quantification of hybridization signals (silver grains).

Main Results:

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  • Calbindin-D28k mRNA levels were significantly higher (over 350%) in distal tubules compared to proximal tubules and glomeruli.
  • Distal tubule cells showed specific hybridization signals, indicating them as the primary site of expression.
  • Results correlated with previous immunocytochemical localization of the protein.

Conclusions:

  • The distal tubule is the principal site of calbindin-D28k gene expression in the murine nephron.
  • This finding supports the role of calbindin-D28k in the function of distal tubule cells.
  • In situ hybridization is effective for localizing specific mRNA expression in kidney tissues.