Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Mass Spectrometry: Overview

Mass Spectrometry: Overview

Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The seminal proteome of a monandrous fly, Drosophila subobscura.

Proceedings. Biological sciences·2026

Same author

Translational bottlenecks in blood-based proteomics.

EMBO molecular medicine·2026

Same author

Author Correction: Community benchmarking and evaluation of human unannotated microprotein detection by mass spectrometry based proteomics.

Nature communications·2026

Same author

Hepatic cellular stress response pathways exhibit species differences in basal and inducible activity.

Toxicological sciences : an official journal of the Society of Toxicology·2026

Same author

A narrow developmental window defines PKN2's essential role in ventricular chamber morphogenesis.

Communications biology·2026

Same author

Expanding the human proteome with microproteins and peptideins.

Nature·2026

Same journal

Transpulmonary proteomic gradient analysis in women with pulmonary arterial hypertension associated with systemic sclerosis.

Journal of proteomics·2026

Same journal

Multiomic insights into fungal polylactic acid degradation: Metabolic adaptation and hydrolytic mechanisms of Sporobolomyces pararoseus.

Journal of proteomics·2026

Same journal

Temporal proteomic analysis reveals a three-phase adaptation strategy in Phytophthora cinnamomi during salinity stress.

Journal of proteomics·2026

Same journal

Proteomic and phosphoproteomic profiles of time-dependent dynamic changes in LPS-induced macrophage polarization.

Journal of proteomics·2026

Same journal

From prediction to mechanism: Explainable AI uncovers plasma and CSF proteomic signatures of Alzheimer's disease.

Journal of proteomics·2026

Same journal

Twenty years of the Mexican Proteomics Society.

Journal of proteomics·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 13, 2026

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

Published on: August 19, 2025

Guidelines for reporting quantitative mass spectrometry based experiments in proteomics.

Salvador Martínez-Bartolomé¹, Eric W Deutsch, Pierre-Alain Binz

¹Proteomics Facility, Centro Nacional de Biotecnología-Consejo Superior de Investigaciones Científicas (CNB-CSIC), ProteoRed ISCIII, Madrid, Spain.

Journal of Proteomics

|March 19, 2013

Summary

This summary is machine-generated.

New guidelines standardize quantitative proteomics data from mass spectrometry. These MIAPE Quant guidelines ensure critical analysis and reproducibility of proteomics studies.

More Related Videos

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

Published on: April 18, 2025

Standards for Quantitative Metalloproteomic Analysis Using Size Exclusion ICP-MS

Standards for Quantitative Metalloproteomic Analysis Using Size Exclusion ICP-MS

Published on: April 13, 2016

Related Experiment Videos

Last Updated: May 13, 2026

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

Published on: August 19, 2025

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

Published on: April 18, 2025

Standards for Quantitative Metalloproteomic Analysis Using Size Exclusion ICP-MS

Standards for Quantitative Metalloproteomic Analysis Using Size Exclusion ICP-MS

Published on: April 13, 2016

Area of Science:

Proteomics
Mass Spectrometry
Data Standardization

Background:

Mass spectrometry is a key tool for protein identification and quantitative proteomics.
Methodological advancements enable large-scale protein abundance measurements.
Various quantitative strategies exist, each with unique data analysis needs.

Purpose of the Study:

To introduce the MIAPE Quant guidelines for reporting quantitative mass spectrometry data.
To standardize data handling, representation, and sharing in proteomics.
To facilitate critical analysis and reproducibility of quantitative proteomics experiments.

Main Methods:

Development of Minimum Information About a Proteomics Experiment (MIAPE) Quant guidelines by the HUPO Proteomics Standards Initiative (HUPO-PSI).
Creation of the mzQuantML data exchange format.
Inclusion of labeled, label-free, and targeted approaches like Selected Reaction Monitoring (SRM).

Main Results:

The MIAPE Quant guidelines define essential data and metadata for quantitative proteomics.
These guidelines support data submission to databases and journals.
The guidelines aim for broad adoption by journals and public proteomics databases.

Conclusions:

The MIAPE Quant guidelines represent a consensus view for reporting quantitative proteomics data.
Standardization through these guidelines will improve data quality and reproducibility.
Adoption is expected to impact proteomics laboratories globally, enhancing data sharing and analysis.