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Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Human Virome01:26

Human Virome

The human body harbors a vast and diverse viral community known as the human virome. The virome includes bacteriophages that infect bacteria, and eukaryotic viruses that infect human cells. Transient dietary and environmental viruses also contribute to this dynamic ecosystem. Estimates suggest the human body may contain on the order of 10¹³ viral particles, though abundance varies widely by body site and detection method.Comprehensive characterization of the virome has become possible only with...
Compounds Essential to Human Function01:25

Compounds Essential to Human Function

The human body is composed of cells that are fundamentally made up of several different molecules. These molecules are essential to carry out all physiological processes in the body and are broadly classified into organic and inorganic based on their chemical structures.
Inorganic Compounds Essential to Human Functioning
Inorganic compounds essential to human functioning include water, salts, acids, and bases. These compounds are inorganic, i.e., they do not have a carbon-hydrogen bond. Water...
Lipid-derived Compounds in the Human Body01:31

Lipid-derived Compounds in the Human Body

Fats and lipids are crucial components in the human body. Some lipid-derived compounds, such as fat-soluble vitamins, eicosanoids, lipoproteins, and glycolipids, also play unique roles to support various  biological processes .
Fat-soluble Vitamins
Fat-soluble vitamins, including vitamins A, D, E, and K, are required in minimal quantities, but their deficiencies can lead to severely abnormal physiological conditions. For example, vitamin A deficiency can cause night blindness, dry skin, delayed...
Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
Proteins: From Genes to Degradation02:11

Proteins: From Genes to Degradation

Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick.  Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
Transcription is the synthesis of RNA molecules by RNA...

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Related Experiment Video

Updated: May 13, 2026

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames
07:38

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames

Published on: April 11, 2019

The human flavoproteome.

Wolf-Dieter Lienhart1, Venugopal Gudipati, Peter Macheroux

  • 1Graz University of Technology, Institute of Biochemistry, Petersgasse 12, A-8010 Graz, Austria.

Archives of Biochemistry and Biophysics
|March 19, 2013
PubMed
Summary

Vitamin B2 (riboflavin) is crucial for making active coenzymes FMN and FAD. Many flavoproteins, essential for metabolism and health, are linked to genetic disorders.

Area of Science:

  • Biochemistry
  • Human Physiology
  • Genetics

Background:

  • Vitamin B2 (riboflavin) is vital for synthesizing flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN).
  • The human genome encodes numerous flavin-dependent proteins involved in critical cellular processes.
  • Flavoproteins utilize FMN or FAD, playing roles in metabolism, cofactor biosynthesis, and hormone regulation.

Purpose of the Study:

  • To detail the functions of flavoenzymes in human health and disease.
  • To highlight the significance of flavin-dependent proteins in metabolic pathways.
  • To discuss the implications of genetic variants in flavin-dependent proteins.

Main Methods:

  • Review of literature on flavin-dependent proteins and their roles.
  • Analysis of gene encoding for flavin-dependent proteins and their functions.

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A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes
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Last Updated: May 13, 2026

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames
07:38

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames

Published on: April 11, 2019

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes
09:10

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes

Published on: May 22, 2018

  • Discussion of flavoenzymes involved in metabolic pathways and cofactor biosynthesis.
  • Main Results:

    • Flavin-dependent enzymes are central to oxidation-reduction processes in major metabolic pathways.
    • Two-thirds of flavin-dependent proteins are associated with disorders due to allelic variants.
    • Flavoproteins are essential for synthesizing cofactors like coenzyme A and hormones such as thyroxine.

    Conclusions:

    • Flavin-dependent proteins are critical for human health, impacting metabolism, biosynthesis, and hormonal regulation.
    • Genetic variations in flavin-dependent proteins contribute to various human diseases.
    • Understanding flavoenzyme function is key to addressing health and disease related to riboflavin metabolism.