Optimization and characterization of polysaccharide-based bioflocculant produced by Paenibacillus elgii B69 and its application in wastewater treatment

The optimization, purification and characterization of bioflocculant produced by Paenibacillus elgii B69 were investigated. The bioflocculant was an exopolysaccharide composed of glucose, glucuronic acid, mannose and xylose. The maximum bioflocculant production was about 25.63 g/L achieved with sucrose at 51.35 g/L, peptone at 6.78 g/L and yeast extract at 0.47 g/L optimized by response-surface methodology. In addition, a series of experiments was performed to investigate the flocculation activities towards kaolin clay, dyeing pigment, heavy metal ion, and real wastewater and the result indicated the new bioflocculant had high activities towards all the tested pollutions. These results showed its great potential for water pretreatment used in industry.