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The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective
Published on: June 16, 2022
Feeding strategies for enhanced lactobionic acid production from whey by Pseudomonas taetrolens
Saúl Alonso1, Manuel Rendueles, Mario Díaz
1Department of Chemical Engineering and Environmental Technology, University of Oviedo, Faculty of Chemistry, C/Julián Clavería s/n, 33071 Oviedo, Spain.
Abstract:
High-level production of lactobionic acid from whey by Pseudomonas taetrolens under fed-batch fermentation was achieved in this study. Different feeding strategies were evaluated according to the physiological status and fermentation performance of P. taetrolens. A lactobionic acid titer of 164 g/L was obtained under co-feeding conditions affording specific and volumetric productivities of 1.4 g/g h and 2.05 g/L h, respectively. Flow cytometry assessment revealed that P. taetrolens cells exhibited a robust physiological status, which makes them particularly well-suited for employing concentrated nutrient solutions to further prolong the growth and production phases. Such detailed knowledge of the physiological status has been revealed to be a key issue to further support the development of high-yield lactobionic acid production processes under feeding strategies. The present study has demonstrated the feasibility of P. taetrolens to achieve high-level bio-production of lactobionic acid from whey through fed-batch cultivation, suggesting its major potential for industrial-scale implementation.
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