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Detecting and quantifying mites in domestic dust: a novel application for real-time PCR

Sandrine Roussel1, Gabriel Reboux, Alexandre Naegele

  • 1Department of Parasitology-Mycology, University Hospital of Besançon, 3 Boulevard Fleming, 25030 Besancon, France. sroussel@univ-fcomte.fr

Environment International
|March 19, 2013
PubMed
Abstract

No abstract available in PubMed .

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Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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