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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles
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Carbon nanoparticle-based fluorescent bioimaging probes.

Susanta Kumar Bhunia1, Arindam Saha, Amit Ranjan Maity

  • 1Centre for Advanced Materials, Indian Association for Cultivation of Science, Kolkata-700032, India.

Scientific Reports
|March 19, 2013
PubMed
Summary
This summary is machine-generated.

Researchers developed novel, non-toxic fluorescent carbon nanoparticles for biological imaging. These carbon nanoparticles offer a safer alternative to heavy metal-based probes for medical diagnostics and cell staining.

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Materials Science

Background:

  • Fluorescent nanoparticle probes enhance biological labeling and medical diagnostics due to superior brightness and photostability.
  • Heavy metal-based semiconductor nanocrystals show promise but face toxicity limitations.
  • Developing non-toxic alternatives is crucial for broader clinical applications.

Purpose of the Study:

  • To develop a non-toxic, fluorescent carbon nanoparticle-based imaging probe.
  • To create a safer alternative to heavy metal-containing probes for biological staining and diagnostics.
  • To explore the potential of carbon nanoparticles in advanced medical imaging.

Main Methods:

  • A chemical synthesis method was employed to create highly fluorescent carbon nanoparticles.
  • The synthesized carbon nanoparticles range from 1-10 nm in size.
  • Functionalization of carbon nanoparticles into nanoprobes with hydrodynamic diameters of 5-15 nm was performed.

Main Results:

  • Highly fluorescent carbon nanoparticles (1-10 nm) were successfully synthesized.
  • The carbon nanoparticles exhibit size-dependent, tunable visible emission.
  • Functionalized nanoprobes were utilized effectively for cell imaging.

Conclusions:

  • Fluorescent carbon nanoparticles present a viable, non-toxic alternative to heavy metal-based probes.
  • These carbon nanoparticles are suitable for biological staining and diagnostics.
  • The developed probes show potential for advancing medical imaging technologies.