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Size-Exclusion Chromatography

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
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A Microfluidic-based Hydrodynamic Trap for Single Particles

A Microfluidic-based Hydrodynamic Trap for Single Particles

Published on: January 21, 2011

Spiral microchannel with rectangular and trapezoidal cross-sections for size based particle separation.

Guofeng Guan¹, Lidan Wu, Ali Asgar Bhagat

¹Department of Mechanical Engineering, National University of Singapore, Singapore.

Scientific Reports

|March 19, 2013

Summary

This summary is machine-generated.

This study introduces a novel 3D particle focusing method in spiral microfluidic systems. A new trapezoidal channel design enhances particle separation resolution by leveraging stronger Dean vortices.

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Area of Science:

Microfluidics
Particle Imaging
Separation Science

Background:

Spiral microfluidic systems are used for particle manipulation.
Understanding particle focusing mechanisms is crucial for effective separation.
Previous methods lacked detailed 3D observation of particle streams.

Purpose of the Study:

To develop a 3D observation method for particle streams in spiral microfluidic channels.
To investigate particle focusing behavior along the channel depth and width.
To design and validate a novel trapezoidal spiral microchannel for enhanced particle separation.

Main Methods:

Three-dimensional observation of focused particle streams.
Analysis of particle focusing mechanisms based on force balance.
Fabrication and experimental testing of a novel trapezoidal cross-section spiral microchannel.
Particle separation resolution assessment.

Main Results:

Particles focus near the top and bottom walls of the microchannel.
A novel trapezoidal channel design generates stronger Dean vortices.
Particle focusing is sensitive to particle size and flow rate.
A size-dependent critical flow rate causes a sharp transition in equilibrium positions.
Enhanced separation resolution compared to rectangular channels.

Conclusions:

The 3D observation method provides clear insights into particle focusing.
The trapezoidal spiral microchannel design improves particle separation resolution.
Particle size and flow rate are key parameters for separation in the novel device.