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Related Experiment Video

Updated: May 13, 2026

Simple, Affordable, and Modular Patterning of Cells using DNA

Simple, Affordable, and Modular Patterning of Cells using DNA

Published on: February 24, 2021

Barcoding cells using cell-surface programmable DNA-binding domains.

Prashant Mali¹, John Aach, Je-Hyuk Lee

¹Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA.

|March 19, 2013

Summary

This summary is machine-generated.

We developed surface zinc fingers (sZFs) to barcode living cells using DNA sequences. This method allows for precise cell labeling and tracking in complex biological systems.

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Area of Science:

Molecular Biology
Cell Biology
Biotechnology

Background:

Cellular barcoding is crucial for understanding complex biological systems.
Existing methods often lack specificity or scalability for multiplexed analysis.

Purpose of the Study:

To introduce a novel cell-surface protein-based barcoding system.
To enable sequence-specific labeling and multiplexed imaging of living cells.

Main Methods:

Engineered programmable zinc-finger DNA-binding domains (sZFs) for cell-surface expression.
Developed sequential labeling strategies using sZFs and distinct DNA-binding pairs.
Utilized fluorescence microscopy for imaging labeled cells.

Main Results:

Demonstrated sequence-specific labeling of living cells with double-stranded DNA (dsDNA) using sZFs.
Achieved multiplexed imaging of over three cell types using three fluorophores via sequential labeling.
Showcased sZF versatility as surrogate reporters, cell capture reagents, and for targeted viral delivery.

Conclusions:

Surface zinc fingers offer a versatile and programmable platform for cellular barcoding.
This technology facilitates advanced applications in cell tracking, diagnostics, and therapeutics.