Related Experiment Video

Updated: May 13, 2026

Orthotopic Transplantation of Syngeneic Lung Adenocarcinoma Cells to Study PD-L1 Expression
06:03

Orthotopic Transplantation of Syngeneic Lung Adenocarcinoma Cells to Study PD-L1 Expression

Published on: January 19, 2019

Tumor suppressor in lung cancer-1 (TSLC1) mediated by dual-regulated oncolytic adenovirus exerts specific antitumor

Wen Lei1, Hong-bin Liu, Shi-bing Wang

  • 1Xinyuan Institute of Medicine and Biotechnology, College of Biological Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China.

Acta Pharmacologica Sinica
|March 19, 2013
PubMed
Abstract

Insights

This study shows that the oncolytic adenovirus Ad·sp-E1A(Δ24)-TSLC1 effectively targets and suppresses non-small cell lung cancer (NSCLC) cell viability and tumor growth. The engineered virus demonstrates significant antitumor activity and increased survival rates in preclinical models.

Area of Science:

  • Oncolytic virotherapy
  • Molecular oncology
  • Gene therapy for cancer

Background:

  • Tumor suppressor in lung cancer-1 (TSLC1) is frequently inactivated in non-small cell lung cancer (NSCLC).
  • Oncolytic adenoviruses offer a targeted approach for cancer treatment.

Purpose of the Study:

  • To investigate the antitumor effects of a dual-regulated oncolytic adenovirus (Ad·sp-E1A(Δ24)-TSLC1) expressing TSLC1 in lung cancer.
  • To elucidate the underlying mechanisms of its antitumor actions.

Main Methods:

  • Construction of the recombinant Ad·sp-E1A(Δ24)-TSLC1 virus.
  • Evaluation of antitumor effects in NSCLC cell lines (NCI-H460, A549, H1299) and a xenograft model.
  • Assessment of cell viability, apoptosis, caspase activation, and viral propagation in tumor tissues.

Main Results:

  • Ad·sp-E1A(Δ24)-TSLC1 induced high TSLC1 expression and selectively suppressed lung cancer cell viability.
  • The virus triggered apoptosis via caspase-8, caspase-3, and PARP activation in cancer cells.
  • Significant tumor volume reduction and increased survival rates were observed in xenograft models, with evidence of apoptosis and viral propagation.

Conclusions:

  • The oncolytic adenovirus Ad·sp-E1A(Δ24)-TSLC1 demonstrates specific antitumor efficacy against lung cancer.
  • This engineered virus represents a promising therapeutic agent for lung cancer treatment.

More Related Videos

Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer06:51

Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer

Published on: July 21, 2018

Using Micro-computed Tomography for the Assessment of Tumor Development and Follow-up of Response to Treatment in a Mouse Model of Lung Cancer11:31

Using Micro-computed Tomography for the Assessment of Tumor Development and Follow-up of Response to Treatment in a Mouse Model of Lung Cancer

Published on: May 20, 2016

Related Concept Videos

Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
Tumor Immunotherapy01:27

Tumor Immunotherapy

Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.