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Real-Time, Two-Color Stimulated Raman Scattering Imaging of Mouse Brain for Tissue Diagnosis

Real-Time, Two-Color Stimulated Raman Scattering Imaging of Mouse Brain for Tissue Diagnosis

Published on: February 1, 2022

Multicolor stimulated Raman scattering (SRS) microscopy.

Fa-Ke Lu¹, Minbiao Ji, Dan Fu

¹Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, United States.

Molecular Physics

|March 19, 2013

Summary

This study introduces a new stimulated Raman scattering (SRS) microscopy method for simultaneous multicolor chemical imaging. The technique enables rapid, high-sensitivity mapping of protein and lipid distributions using CH2 and CH3 signals.

Keywords:

coherent Raman scattering grating lipid lock-in mouse skin multicolor poly(methyl methacrylate)polystyrene protein pulse shaper stimulated Raman scattering

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Area of Science:

Biomedical Optics
Chemical Imaging
Microscopy

Background:

Stimulated Raman scattering (SRS) microscopy enables biochemical imaging by targeting specific molecular vibrations.
Conventional SRS microscopy with picosecond pulses is limited to single Raman band chemical mapping.
There is a need for advanced SRS techniques capable of multicolor imaging for comprehensive biochemical analysis.

Purpose of the Study:

To develop a novel SRS microscopy technique for simultaneous multicolor imaging.
To enhance sensitivity and acquisition speed in SRS chemical mapping.
To demonstrate the simultaneous mapping of protein and lipid distributions.

Main Methods:

Implementation of a grating-based pulse shaper for excitation.
Utilization of a grating-based spectrograph for detection.
Application of linear combination analysis on CH2 and CH3 stretching signals for molecular distribution mapping.

Main Results:

Achieved simultaneous multicolor SRS imaging with high sensitivity.
Demonstrated high acquisition speeds for chemical mapping.
Successfully mapped the distributions of protein and lipid contents concurrently.

Conclusions:

The developed SRS microscopy technique overcomes limitations of single-band imaging.
This method allows for simultaneous, high-speed, and sensitive multicolor biochemical analysis.
The technique provides a powerful tool for visualizing the spatial distribution of key biomolecules like proteins and lipids.