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Updated: May 13, 2026

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028
Published on: January 13, 2017
A trans-acting ketoreductase in biosynthesis of a symmetric polyketide dimer SIA7248
Yi Zou1, Haixing Yin, Dekun Kong
1State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030, P.R. China.
Abstract:
NEW ASSEMBLY LINE, NEW COMPOUND: SIA7248, a new symmetric macrolide, was isolated from a marine-derived Streptomyces strain. Bioinformatic analyses of the identified biosynthetic gene cluster (sia) for SIA7248 suggested a polyketide biosynthesis utilizing an iteratively trans-acting ketoreductase (KR). We characterized SiaM as a trans-KR to catalyse reductions of various β-ketoacyl-thioesters with D-stereospecificity.
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