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Updated: May 13, 2026
Humanized Mediator Release Assay as a Read-Out for Allergen Potency
Published on: June 29, 2021
Toll-like receptor-expressing cells for antiallergy compound screening
1INSERM, Institut National de la Santé et de la Recherche Médicale U774, 59019 Lille, France.
Background:
Regulation of type 2 helper T cell (TH2) polarization by toll-like receptors (TLRs) has triggered great interest in new antiallergic therapeutics. In addition to being involved in the regulation of co-stimulation by antigen-presenting cells, they are expressed on other immune and non-immune cells.
Objective:
To review the expression and function of TLRs on these cells and their potential to regulate TH2-associated responses.
Methods:
We focused on human cells that can be used for in vitro testing of TLR agonists.
Results/Conclusion:
Many cells involved in the allergic reaction have the capacity to respond to TLR agonists. Therefore, one needs to be cautious in extrapolating the antiallergic effect of a TLR agonist from the response analyzed in one cell type. Therefore, it is suggested that several cell types should be studied.
