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Related Experiment Videos

Histone variant patterns during vertebrate embryogenesis and limb development.

A M Wunsch1, J Lough

  • 1Department of Anatomy and Cellular Biology, Medical College of Wisconsin, Milwaukee 53226.

Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists
|April 1, 1990
PubMed
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Chicken embryo development involves stable histone variant ratios but increasing H2A ubiquitination during hindlimb formation. This suggests post-translational modifications play a key role in developmental processes.

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Epigenetics

Background:

  • Core histone variants are crucial for chromatin structure and gene regulation.
  • Understanding histone dynamics during embryogenesis provides insights into developmental processes.
  • Avian histone variants, including H3.3, have been identified but their developmental roles require further investigation.

Purpose of the Study:

  • To investigate the relative content of core histone variants during early chicken embryogenesis.
  • To analyze changes in histone variant composition during chicken hindlimb development.
  • To determine the extent of H2A ubiquitination during embryonic hindlimb development.

Main Methods:

  • Two-dimensional gel electrophoresis was employed to separate and quantify histone variants.

Related Experiment Videos

  • Nuclei from chicken embryos at various developmental stages (Stage 1, 19, and 29) were analyzed.
  • Relative abundance of core histone variants and ubiquitinated H2A conjugates was assessed.

    • Main Results:

    • All known avian histone variants, including H3.3, were detected at Stage 1 of embryogenesis.
    • The ratios of core histone variants remained constant from early embryogenesis (Stage 1) through hindlimb development (Stage 29).
    • The proportion of ubiquitinated H2A variants significantly increased from 4.5% at Stage 19 to 12% at Stage 29 of hindlimb development.

    Conclusions:

    • Core histone variant composition is largely stable during early chicken embryogenesis and hindlimb development.
    • Post-translational modification, specifically H2A ubiquitination, increases significantly during chicken hindlimb development.
    • These findings highlight the dynamic nature of histone modifications in regulating developmental processes, even when core variant ratios are constant.