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Updated: May 13, 2026

Targeted DNA Methylation Analysis by Next-generation Sequencing

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

Genome-wide DNA methylation analysis using massively parallel sequencing technologies.

Masako Suzuki¹, John M Greally

¹Center for Epigenomics, Department of Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA. masako.suzuki@einstein.yu.edu

Seminars in Hematology

|March 20, 2013

Summary

This summary is machine-generated.

This review covers genome-wide DNA methylation profiling techniques, discussing their strengths and weaknesses. It also explores emerging DNA modifications and detection protocols for epigenetic research.

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Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

Area of Science:

Molecular Biology
Genetics
Epigenetics

Background:

Epigenetics involves heritable changes in gene expression without altering DNA sequence.
DNA methylation is a key epigenetic modification crucial for development and disease.
Genome-wide DNA methylation profiling is an important epigenomic assay.

Purpose of the Study:

To review protocols for genome-wide DNA methylation profiling using massively parallel sequencing (MPS).
To discuss the strengths and weaknesses of current MPS-based DNA methylation profiling methods.
To describe newly discovered DNA modifications and their detection protocols.

Main Methods:

Massively parallel sequencing (MPS) techniques for genome-wide DNA methylation profiling.
Comparative analysis of strengths and weaknesses of various MPS protocols.
Description of methods for detecting novel DNA modifications.

Main Results:

Evaluation of established and emerging protocols for DNA methylation analysis.
Identification of advantages and limitations of different MPS techniques.
Overview of methods for characterizing new epigenetic marks.

Conclusions:

Understanding the nuances of MPS protocols is vital for accurate epigenomic profiling.
Ongoing research into novel DNA modifications expands the scope of epigenetic studies.
Accurate detection of epigenetic modifications is critical for both basic research and clinical applications.