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Decellularized Apple-Derived Scaffolds for Bone Tissue Engineering In Vitro and In Vivo
09:49

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Published on: February 23, 2024

Combining technologies to create bioactive hybrid scaffolds for bone tissue engineering.

Anandkumar Nandakumar1, Ana Barradas1, Jan de Boer1

  • 1Department of Tissue Regeneration; MIRA Institute for Biomedical Technology and Technical Medicine; University of Twente; Enschede, The Netherlands.

Biomatter
|March 20, 2013
PubMed
Summary

Engineered polymer-ceramic scaffolds using rapid prototyping and electrospinning show promise for bone regeneration. Combining fabrication methods and biomimetic coatings influences cell behavior and osteogenic gene expression.

Keywords:
biomimetic coatingbone tissue engineeringcalcium-phosphateelectrospinningpolymerrapid prototyping

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Tissue engineering seeks scaffolds mimicking bone's organic and inorganic phases.
  • Combining physical and chemical cues enhances cell response.
  • Polymer-ceramic hybrids offer tunable properties for bone regeneration.

Purpose of the Study:

  • To fabricate and characterize polymer-ceramic hybrid scaffolds for bone regeneration.
  • To evaluate the effect of combined fabrication techniques (3D fiber deposition and electrospinning) and biomimetic coating on cell behavior.
  • To assess the potential of these scaffolds in promoting osteogenesis.

Main Methods:

  • Fabrication of scaffolds using 3D fiber deposition and electrospinning of PEOT/PBT block copolymer.
  • Biomimetic coating with a calcium-phosphate (Ca-P) layer.
  • Characterization using SEM, EDX, and FTIR.
  • In vitro culture of human mesenchymal stromal cells (hMSCs) on scaffolds.
  • Assessment of cell attachment, proliferation, ECM secretion, and osteogenic gene expression.

Main Results:

  • Scaffolds exhibited suitable morphology and composition.
  • hMSCs attached, proliferated, and secreted ECM on all scaffolds.
  • Coated 3DF scaffolds showed higher DNA content in basic medium, while coated 3DF+ESP scaffolds had decreased DNA in mineralization medium at 21 days.
  • Combined technologies and coating affected osteogenic marker expression (cbfa1, BMP-2, OP, OC, ON).

Conclusions:

  • The combined fabrication approach (3DF deposition and electrospinning) with biomimetic coating shows potential for bone tissue engineering.
  • Scaffold design and coating influence cellular response and osteogenic differentiation.
  • Further investigation is warranted to optimize these hybrid scaffolds for enhanced bone regeneration.