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Related Experiment Video

Updated: May 13, 2026

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

Highly sensitive grating coupler-based surface plasmon-coupled emission (SPCE) biosensor for immunoassay.

Jong Seol Yuk¹, Ernest F Guignon, Michael A Lynes

¹Department of Molecular and Cell Biology, University of Connecticut, 91 North Eagleville Road, Storrs, CT 06269-3125, USA.

|March 20, 2013

Summary

This summary is machine-generated.

This study optimized a novel surface plasmon-coupled emission (SPCE) biosensor for high-content analysis. The optimized polyelectrolyte spacer layer enhances sensitivity, achieving a 1 pg/mL limit of detection for immunoassays.

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Last Updated: May 13, 2026

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

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Published on: March 17, 2023

Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum

Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum

Published on: December 13, 2017

Area of Science:

Biosensing technologies
Surface plasmon resonance (SPR)
High-content analysis

Background:

Surface plasmon-coupled emission (SPCE) biosensors offer sensitive detection.
Polyelectrolyte (PEL) layers are used to optimize biosensor performance.
Optimizing PEL spacer layers is crucial for minimizing signal quenching and maximizing detection.

Purpose of the Study:

To evaluate a novel high-content gold grating coupler-based SPCE biosensor system.
To optimize the polyelectrolyte (PEL) spacer layer for enhanced SPCE response.
To assess the biosensor's performance in direct and sandwich immunoassay formats.

Main Methods:

Utilized layer-by-layer (LbL) deposition to create PEL spacer layers on a gold surface.
Characterized PEL spacer layers using surface plasmon resonance (SPR) and ellipsometry.
Evaluated SPCE response with varying spacer layer thicknesses and immunoassay formats.

Main Results:

SPCE response decreased with spacer layer thickness above 10 nm due to quenching.
An optimized two-layer PEL spacer (poly(diallyldimethylammonium chloride)/poly(acrylic acid)) minimized quenching and maximized SPCE.
A mouse IgG sandwich immunoassay demonstrated a dose-dependent response with a 1 pg/mL limit of detection.
Analyzed a nine-log dynamic range of human IgG concentrations in human serum.

Conclusions:

The optimized PEL spacer layer significantly enhances SPCE biosensor sensitivity.
The grating coupler-based SPCE biosensor is a promising platform for sensitive high-content analysis.
This optimized biosensor system has potential applications in various immunoassay formats and biological sample analysis.