Related Experiment Video
Updated: May 13, 2026
09:35
A Mouse Model of Hemorrhagic Transformation Induced by Acute Hyperglycemia Combined with Transient Focal Ischemia
Published on: November 15, 2024
Hyperglycemia increases susceptibility to ischemic necrosis.
D Lévigne1, M Tobalem, A Modarressi
1Division of Plastic, Reconstructive and Aesthetic Surgery, University Hospitals of Geneva and Faculty of Medicine, University of Geneva, Rue Gabrielle-Perret-Gentil 4, 1211 Geneva 14, Switzerland.
Biomed Research International
|March 20, 2013
Summary
Hyperglycemia, or high blood sugar, significantly increases the risk of limb necrosis in diabetic patients experiencing critical limb ischemia. Strict glycemic control is crucial for managing these severe diabetic wounds.
Area of Science:
- Biomedical research
- Diabetology
- Vascular surgery
Background:
- Diabetic foot ulcers are a major complication, leading to necrosis.
- Arteriosclerosis and neuropathy are implicated, but mechanisms of necrosis are unclear.
- Hyperglycemia's direct role in ischemic tissue necrosis requires investigation.
Purpose of the Study:
- To investigate the effect of hyperglycemia on ischemic tissue necrosis.
- To determine if hyperglycemia exacerbates necrosis independent of other diabetic complications.
Main Methods:
- A critical hind limb ischemia model was used in rats.
- Rats were made hyperglycemic (streptozotocin) or euglycemic.
- Ischemia severity and skin perfusion (laser Doppler) were standardized.
Main Results:
- Both groups experienced similar reductions in skin perfusion.
- Hyperglycemic rats showed a significantly higher rate of limb necrosis (71% vs. 29%).
- Hyperglycemia per se increases susceptibility to necrosis under ischemic conditions.
Conclusions:
- Hyperglycemia directly worsens ischemic tissue necrosis.
- Findings highlight the importance of glycemic control in diabetic patients with critical limb ischemia.
- This may improve understanding of diabetic wound progression.
Related Concept Videos
01:29Hyperglycemia
Hyperglycemia is an abnormally high blood glucose level. It is diagnosed by fasting glucose ≥126 mg/dL, 2-hour oral glucose tolerance test (or OGTT) ≥200 mg/dL, random glucose ≥200 mg/dL with symptoms, or HbA1c ≥6.5%. However, HbA1c results may be unreliable in certain conditions, such as anemia or hemoglobinopathies, and the diagnosis should be confirmed unless classic symptoms are present. Postprandial hyperglycemia is typically considered significant when glucose levels exceed 180 mg/dL two...
01:26Hypoglycemia
Hypoglycemia is a blood glucose level below 70 mg/dL. It commonly occurs in individuals using insulin or insulin-secreting drugs, but may also arise in non-diabetic conditions. People with type 1 diabetes are at the highest risk because they depend on exogenous insulin. People with type 2 diabetes are also at risk, especially when treated with insulin or medications such as sulfonylureas, which increase insulin release regardless of blood glucose levels. It develops when insulin levels exceed...
01:22Diabetic Neuropathy
DefinitionDiabetic neuropathy is nerve damage caused by long-standing diabetes mellitus. It results directly from prolonged high blood sugar levels.PathophysiologyThe pathophysiology of diabetic neuropathy involves both metabolic and vascular disturbances triggered by chronic hyperglycemia.Metabolic injury: Elevated glucose levels activate the polyol pathway within nerve cells, leading to the accumulation of sorbitol and fructose. This increases oxidative stress, disrupts normal nerve...
01:15Hypoglycemia and Glucagon
Without prolonged fasting, healthy individuals maintain blood glucose levels above 3.5 mM due to a well-adapted neuroendocrine counterregulatory system that effectively prevents acute hypoglycemia, a potentially life-threatening condition. The primary clinical scenarios for hypoglycemia encompass diabetes treatment, inappropriate production of endogenous insulin or insulin-like substances by tumors, and the use of glucose-lowering agents in non-diabetic individuals. Notably, hypoglycemia in the...
01:22Complications of Diabetes Mellitus
Diabetes mellitus is a chronic metabolic disorder characterized by persistent hyperglycemia due to insulin deficiency, resistance, or both. Prolonged hyperglycemia disrupts metabolic homeostasis and leads to acute and chronic complications.Acute ComplicationsAcute complications result from sudden metabolic imbalance.Diabetic ketoacidosis (DKA) mainly appears in type 1 diabetes but may also develop in type 2 diabetes, particularly under extreme stress. It arises from severe insulin deficiency,...
01:24Type II Diabetes II: Pathophysiology
PathophysiologyType 2 diabetes mellitus (T2DM ) is a chronic metabolic disorder characterized by insulin resistance and progressive pancreatic β-cell dysfunction, leading to impaired glucose homeostasis. It results from interactions among genetic predisposition, environmental factors, and metabolic stressors, such as overnutrition and a sedentary lifestyle.Insulin Resistance and Glucose DysregulationEarly T2DM involves insulin resistance in skeletal muscle, adipose tissue, and the liver.