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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

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Related Experiment Video

Updated: May 13, 2026

Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy
08:30

Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy

Published on: July 18, 2011

Mapping of surface-immobilized DNA with force-based atomic force microscopy.

Yoonhee Lee1, Sung Hong Kwon, Youngkyu Kim

  • 1Department of Chemistry, Pohang University of Science and Technology, San 31 Hyoja-dong, Pohang, 790-784, South Korea.

Analytical Chemistry
|March 21, 2013
PubMed
Summary

Atomic force microscopy revealed DNA cluster size increases with length. Pulling angle affects measurements, with apparent stretching distance and unbinding force decreasing as the probe moves from the center.

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Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography
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Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography

Published on: October 25, 2018

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Last Updated: May 13, 2026

Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy
08:30

Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy

Published on: July 18, 2011

High-Speed Atomic Force Microscopy Imaging of DNA Three-Point-Star Motif Self Assembly Using Photothermal Off-Resonance Tapping
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High-Speed Atomic Force Microscopy Imaging of DNA Three-Point-Star Motif Self Assembly Using Photothermal Off-Resonance Tapping

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Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography
11:05

Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography

Published on: October 25, 2018

Area of Science:

  • * Biophysics
  • * Nanotechnology
  • * Molecular Biology

Background:

  • * Understanding DNA behavior at the nanoscale is crucial for developing novel biosensors and nanodevices.
  • * Atomic force microscopy (AFM) offers high-resolution imaging and force measurements for single-molecule studies.
  • * Immobilized single-stranded DNA (ssDNA) provides a model system to investigate molecular interactions and structural dynamics.

Purpose of the Study:

  • * To investigate the force-dependent behavior of immobilized single-stranded DNA (ssDNA) of varying lengths.
  • * To analyze the influence of thermal drift and pulling geometry on AFM measurements of DNA.
  • * To quantify the relationship between DNA length and cluster dimensions.

Main Methods:

  • * Immobilization of 50-mer, 100-mer, and 150-mer ssDNAs onto a surface.
  • * Force-based atomic force microscopy (AFM) utilizing a complementary DNA probe on the AFM tip.
  • * Generation of high-resolution topographical maps and analysis of force, stretching distance, unbinding probability, cluster size, and distortion.

Main Results:

  • * Cluster radius demonstrated a power-law relationship with the number of bases (N), proportional to N(0.6) and N(0.53).
  • * Thermal drift caused increased cluster shape distortion at decreased scan speeds and reduced map areas.
  • * Apparent stretching distance and unbinding force were dependent on the pulling angle (sin θ), decreasing with probe displacement from the center.

Conclusions:

  • * DNA cluster size scales predictably with length, offering insights into DNA assembly and organization.
  • * AFM measurement parameters are sensitive to experimental conditions like scan speed and pulling geometry.
  • * The findings provide a quantitative understanding of ssDNA behavior under force, relevant for nanobiotechnology applications.