Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution00:52

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inactivation of PETase at Interfaces Inhibits PET Plastic Depolymerization.

ACS sustainable chemistry & engineering·2026
Same author

Electrokinetic nanoparticle transport in an interconnected porous environment: Decoupling cavity escape and directional bias.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Mixed lipid bilayers enable enhanced stability and activity retention of Lipase A in low-pH environments.

Colloids and surfaces. B, Biointerfaces·2025
Same author

Thermostable Enzyme Variants in the Lower Mevalonate Pathway Improve Isoprenoid Production by Cell-Free Biocatalysis.

ACS sustainable chemistry & engineering·2025
Same author

Anomalous versus Fickian-but-Not-Gaussian Diffusion in Heterogeneous Systems Depends on the Time Scale of Observation.

The journal of physical chemistry letters·2025
Same author

High-Throughput Screening Identifies Anionic Polymer Supports that Improve Enzyme Activity at Low pH and High Temperature.

Langmuir : the ACS journal of surfaces and colloids·2025

Related Experiment Video

Updated: May 13, 2026

Orientational Transition in a Liquid Crystal Triggered by the Thermodynamic Growth of Interfacial Wetting Sheets
06:26

Orientational Transition in a Liquid Crystal Triggered by the Thermodynamic Growth of Interfacial Wetting Sheets

Published on: May 15, 2017

Liquid crystal reorientation induced by aptamer conformational changes.

Patrick S Noonan1, Richard H Roberts, Daniel K Schwartz

  • 1Department of Chemical and Biological Engineering, University of Colorado Boulder , Boulder, Colorado 80309-0424, United States.

Journal of the American Chemical Society
|March 21, 2013
PubMed
Summary
This summary is machine-generated.

Aptamer binding to small molecules triggers liquid crystal reorientation, enabling sensitive, real-time detection. This label-free method shows potential for simultaneous detection of small molecules and nucleic acids.

More Related Videos

Synthetic Condensates and Cell-Like Architectures from Amphiphilic DNA Nanostructures
08:02

Synthetic Condensates and Cell-Like Architectures from Amphiphilic DNA Nanostructures

Published on: May 31, 2024

High-Contrast and Fast Photorheological Switching of a Twist-Bend Nematic Liquid Crystal
06:24

High-Contrast and Fast Photorheological Switching of a Twist-Bend Nematic Liquid Crystal

Published on: October 31, 2019

Related Experiment Videos

Last Updated: May 13, 2026

Orientational Transition in a Liquid Crystal Triggered by the Thermodynamic Growth of Interfacial Wetting Sheets
06:26

Orientational Transition in a Liquid Crystal Triggered by the Thermodynamic Growth of Interfacial Wetting Sheets

Published on: May 15, 2017

Synthetic Condensates and Cell-Like Architectures from Amphiphilic DNA Nanostructures
08:02

Synthetic Condensates and Cell-Like Architectures from Amphiphilic DNA Nanostructures

Published on: May 31, 2024

High-Contrast and Fast Photorheological Switching of a Twist-Bend Nematic Liquid Crystal
06:24

High-Contrast and Fast Photorheological Switching of a Twist-Bend Nematic Liquid Crystal

Published on: October 31, 2019

Area of Science:

  • Biophysics
  • Materials Science
  • Analytical Chemistry

Background:

  • Aptamers are nucleic acid or peptide molecules that bind targets with high specificity.
  • Liquid crystals (LCs) can reorient in response to surface interactions.
  • Real-time detection of small molecules is crucial in various scientific fields.

Purpose of the Study:

  • To investigate aptamer-ligand binding events at a liquid crystal interface.
  • To explore the potential of using liquid crystal reorientation for label-free detection.
  • To determine the sensitivity and selectivity of this detection method.

Main Methods:

  • Utilizing a surfactant-laden aqueous/liquid crystal interface.
  • Observing liquid crystal reorientation in real-time using polarized light.
  • Employing circular dichroism and resonance energy transfer for mechanistic studies.

Main Results:

  • Aptamer-ligand binding induced a detectable liquid crystal reorientation.
  • The response was sensitive, with detection at aptamer dissociation constant concentrations.
  • Control experiments with analogues showed no response, indicating selectivity.
  • Aptamer conformational changes (random coil to secondary structures) correlated with LC reorientation (planar to homeotropic).

Conclusions:

  • Aptamer-ligand binding at an LC interface can induce observable LC reorientation.
  • This phenomenon offers a sensitive and selective platform for label-free detection.
  • The system shows promise for simultaneous detection of small molecules and nucleic acids.