Caffeic acid phenethyl ester improves burn healing in rats through anti-inflammatory and antioxidant effects

Although caffeic acid phenethyl ester (CAPE) has beneficial properties, its anti-inflammatory and antioxidant effects on healing burn injury have not been investigated as yet. Female Wistar rats were divided in two groups: burn and burn + CAPE. A scald injury (burn) was performed. CAPE treatment (10 µmol kg) began immediately after the burn and lasted for 14 days. Euthanasia was performed 14 or 70 days after burning. Seven, 21, and 70 days after burning, burn + CAPE group presented smaller wound area. Increase in reepithelialization was observed in burn + CAPE group 28 and 63 days after burning. Fourteen days after wounding, burn + CAPE group presented diminished myeloperoxidase activity and nitrite levels, reduced CD68 and platelet endothelial cell adhesion molecule 1 protein expression, and less oxidative damage (decrease in malondialdehyde (MDA) and carbonyl levels in plasma and lesion extracts). Seventy days after burning, the amount of myofibroblasts and macrophages (CD68 positive) was decreased and the amount of hydroxyproline was increased in burn + CAPE group. Treatment with CAPE improved burn wound healing, showing decrease in inflammatory parameters and in oxidative damage.