Role of formation of statistical aggregates in chlorophyll fluorescence concentration quenching

Using extensive Monte Carlo simulations, a comprehensive investigation has been carried out on the phenomenon of chlorophyll fluorescence concentration quenching. Our results reveal that statistical aggregations of chlorophylls act mainly as trapping sites for excitation energy and lead to fluorescence quenching. Due to transition dipolar-dipolar interactions between the chlorophylls within a statistical aggregate, the associated oscillator strength changes in comparison to a monomer, and excited energy states show splitting. Further, as the lower energy states are more likely associated with lower oscillator strengths, the fluorescence intensity is observed to decrease. Due to the rapid energy transfer between chlorophyll molecules after photoexcitation, the excitonic energy can easily reach a statistical aggregate, where trapping of the exciton and its subsequent decay occur. With an increase in the chlorophyll concentration, the probability of statistical aggregation increases, thereby accentuating the fluorescence quenching effect.