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Related Experiment Video

Updated: May 13, 2026

Optogenetic Inhibition of Rho1-Mediated Actomyosin Contractility Coupled with Measurement of Epithelial Tension in Drosophila Embryos

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Published on: April 14, 2023

Inversin modulates the cortical actin network during mitosis.

Michael E Werner¹, Heather H Ward, Carrie L Phillips

¹Department of Anatomy and Cell Biology, Indiana University, Indianapolis, IN, USA.

American Journal of Physiology. Cell Physiology

|March 22, 2013

Summary

Inversin protein is crucial for proper cell division and kidney development. Its absence leads to cell division defects and abnormal kidney structures in nephronophthisis type II.

Keywords:

actin cytoskeleton mitosis mitotic spindle polycystic kidney disease

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Aip1p Dynamics Are Altered by the R256H Mutation in Actin

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Published on: July 30, 2014

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Aip1p Dynamics Are Altered by the R256H Mutation in Actin

Aip1p Dynamics Are Altered by the R256H Mutation in Actin

Published on: July 30, 2014

Area of Science:

Cell Biology
Developmental Biology
Genetics

Background:

Mutations in inversin cause nephronophthisis type II, a kidney disease characterized by cyst formation.
Cyst formation suggests a potential role for inversin in planar cell polarity and cell division.

Purpose of the Study:

To investigate the role of inversin in cell division during kidney development.
To understand how inversin regulates the cytoskeleton and cell shape during mitosis.

Main Methods:

Analysis of developing nephrons from inversin-deficient (inv-/-) mouse embryos.
Depletion of inversin using siRNA in cultured mammalian cells.
Microscopy to observe cell morphology, nuclear status, cilia, and spindle organization.
Cell motility assays using fibroblasts from inv-/- mice.

Main Results:

Inv-/- embryos and inversin-depleted cells exhibit increased binucleation and multinucleation.
Loss of inversin perturbs mitotic cell rounding and cortical actin organization, leading to filopodia formation.
Spindle positioning defects result in asymmetric cell division planes.
Inv-/- fibroblasts show reduced migration speed.

Conclusions:

Inversin regulates the cortical actin cytoskeleton, essential for cell rounding and spindle positioning during mitosis.
Defects in cell division, including aberrant spindle orientation and actin organization, contribute to abnormal kidney development in inversin-deficient conditions.