Related Experiment Video
Updated: May 13, 2026
Assessment of Memory Function in Pilocarpine-induced Epileptic Mice
Published on: June 4, 2020
Expression pattern of sorting Nexin 25 in temporal lobe epilepsy: a study on patients and pilocarpine-induced rats
Yingshi Du1, Yan Zou, Weihua Yu
1Department of Geriatrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Purpose:
The transforming growth factor β (TGF-β) signaling pathway is involved in the epileptogenesis. Sorting Nexin 25 (SNX25) has been recently proposed to modulate TGF-β signaling through endosomal sorting of TGF-β receptors for lysosomal degradation. The aim of the present study was to determine the expression pattern of SNX25 in brains of epilepsy patients and in animal model of epilepsy.
Methods:
We investigated the expression of SNX25 in the brain tissues of patients with temporal lobe epilepsy (TLE) and in the pilocarpine-induced rat model of epilepsy using western blotting, real-time quantitative RT-PCR, and double-label immunofluorescence.
Results:
The expression of SNX25 was significantly increased in TLE patients in comparison to controls (0.21±0.07 vs. 0.11±0.03, P<0.05). In the lithium-pilocarpine induced epileptic rats, significant elevation of SNX25 levels was detected in the chronic phase, while no SNX25 alteration occurred in the acute and latent phases. Moreover, SNX25 localized to astrocytes and neurons, in both human samples and animal models.
Conclusion:
Our results indicate that upregulation of SNX25 might be involved in the development of temporal lobe epilepsy.
Insights
Sorting Nexin 25 (SNX25) is upregulated in temporal lobe epilepsy (TLE) patients and in a chronic epilepsy animal model. This suggests SNX25 may play a role in epilepsy development.
Area of Science:
- Neuroscience
- Molecular Biology
- Cell Biology
Background:
- The transforming growth factor β (TGF-β) pathway is implicated in epileptogenesis.
- Sorting Nexin 25 (SNX25) may regulate TGF-β signaling via receptor lysosomal degradation.
Purpose of the Study:
- To investigate the expression pattern of SNX25 in human epilepsy and an epilepsy animal model.
- To explore the potential role of SNX25 in the development of temporal lobe epilepsy (TLE).
Main Methods:
- Western blotting, RT-qPCR, and immunofluorescence were used.
- Brain tissues from TLE patients and pilocarpine-induced epileptic rats were analyzed.
- SNX25 expression and localization were examined in different epilepsy phases.
Main Results:
- SNX25 expression was significantly increased in TLE patients compared to controls.
- Elevated SNX25 levels were observed in the chronic phase of the rat epilepsy model.
- SNX25 was found to localize in both astrocytes and neurons in human and animal samples.
Conclusions:
- Upregulation of SNX25 is associated with temporal lobe epilepsy.
- SNX25 may be a contributing factor in the pathogenesis of TLE.
