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Related Concept Videos

Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Inhibition of CDK Activity02:34

Inhibition of CDK Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
Positive Regulator Molecules02:39

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
Positive Regulator Molecules01:45

Positive Regulator Molecules

To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...

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Related Experiment Video

Updated: May 13, 2026

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

PP2A(Cdc55) regulates G1 cyclin stability.

Paula McCourt1, Christina Gallo-Ebert, Yan Gonghong

  • 1Venenum Biodesign, Genesis Biotechnology Group, Hamilton, NJ, USA.

Cell Cycle (Georgetown, Tex.)
|March 23, 2013
PubMed
Summary

Protein phosphatase 2A (PP2A) is crucial for cell cycle progression. This study reveals PP2A regulates G1-S cyclin stability by controlling their phosphorylation, preventing degradation and ensuring proper cell division.

Keywords:
cell cyclecyclinphosphorylationprotein phosphataseubiquitin

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Related Experiment Videos

Last Updated: May 13, 2026

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
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Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations
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Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations

Published on: September 17, 2012

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
12:02

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Cell cycle progression relies on precise cyclin regulation.
  • Mammalian D-type cyclins drive the G1-S transition.
  • D1 cyclin stability is controlled by SCF E3 ubiquitin ligase-mediated degradation, triggered by phosphorylation.

Purpose of the Study:

  • To investigate the regulation of D-type cyclin phosphorylation.
  • To understand how protein phosphatase 2A (PP2A) influences G1-S cyclin stability.

Main Methods:

  • Utilized S. cerevisiae as a model organism.
  • Investigated the role of PP2A (specifically Cdc55) in regulating the G1 cyclin Cln2.
  • Analyzed the phosphorylation state and stability of Cln2 in wild-type and cdc55-null cells.

Main Results:

  • PP2A(Cdc55) directly regulates Cln2 phosphorylation and stability.
  • Loss of Cdc55 leads to hyperphosphorylation and degradation of Cln2.
  • A non-phosphorylatable Cln2 mutant is stable in cdc55-null cells.
  • cdc55-null cells exhibit inviability when SCF(Grr1) activity is compromised or Cln2 is overexpressed.

Conclusions:

  • PP2A is essential for maintaining G1-S cyclin levels.
  • PP2A modulates cyclin phosphorylation status, which is critical for cell cycle transit.
  • A functional interplay exists between SCF and PP2A in regulating cell cycle progression via cyclin degradation/stability.