Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Modeling and correction of fringe patterns in Doppler asymmetric spatial heterodyne interferometry.

Applied optics·2023

Same author

Absolute characterization of high numerical aperture microscope objectives utilizing a dipole scatterer.

Light, science & applications·2021

Same author

Analysis and correction of distortions in a spatial heterodyne spectrometer system.

Applied optics·2019

Same author

Effective wind and temperature retrieval from Doppler asymmetric spatial heterodyne spectrometer interferograms.

Applied optics·2018

Same author

Simultaneous measurement of phase transmission and birefringence of an object under test.

Applied optics·2018

Same author

Measuring the modulus of the spatial coherence function using an error tolerant phase shifting algorithm and a continuous lateral shearing interferometer.

Optics express·2017

Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026

Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026

Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026

Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026

Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026

Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jan 17, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

Interferometric homogeneity test using adaptive frequency comb illumination.

Klaus Mantel¹, Johannes Schwider

¹Max Planck Institute for the Science of Light, Erlangen, Germany. Klaus.Mantel@mpl.mpg.de

|March 23, 2013

Summary

This summary is machine-generated.

An adaptive frequency comb enables precise homogeneity testing of glass plates using Fizeau interferometry. This method improves fringe visibility for accurate measurements in transmitted and reflected light.

More Related Videos

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

Measurement of X-ray Beam Coherence along Multiple Directions Using 2-D Checkerboard Phase Grating

Measurement of X-ray Beam Coherence along Multiple Directions Using 2-D Checkerboard Phase Grating

Published on: October 11, 2016

Related Experiment Videos

Last Updated: Jan 17, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

Measurement of X-ray Beam Coherence along Multiple Directions Using 2-D Checkerboard Phase Grating

Measurement of X-ray Beam Coherence along Multiple Directions Using 2-D Checkerboard Phase Grating

Published on: October 11, 2016

Area of Science:

Optical metrology
Interferometry
Materials science

Background:

Homogeneity testing of glass plates is crucial for optical applications.
Traditional Fizeau interferometer setups for transmitted light measurements create nested cavities, complicating signal separation.
Existing methods for separating interference signals from nested cavities can suffer from lower fringe visibility.

Purpose of the Study:

To develop a novel method for separating interference signals in Fizeau interferometers with nested cavities.
To enable accurate homogeneity testing of glass plates in both transmitted and reflected light.
To enhance the visibility of interference fringe systems for improved measurement precision.

Main Methods:

Illumination of a Fizeau interferometer with an adaptive frequency comb.
Generation of the adaptive frequency comb using a variable Fabry-Perot filter and a superluminescence diode.
Measurement of glass samples in both reflected and transmitted light within the interferometer.

Main Results:

Successful separation of interference signals from different cavities in the Fizeau interferometer.
Accurate measurement of rigid glass plate homogeneity, including detection of linear variations.
Significantly higher visibility of the fringe system compared to traditional two-beam interferometer filtering approaches.

Conclusions:

The adaptive frequency comb technique offers a superior solution for homogeneity testing of glass plates.
This method enhances measurement accuracy and simplifies the analysis of interference signals in complex optical setups.
The technique is valuable for quality control and characterization in optical manufacturing and research.