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Related Experiment Video

Updated: May 13, 2026

Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues
11:54

Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues

Published on: October 20, 2019

Partial hydatidiform mole: histologic parameters in correlation with DNA genotyping.

Natalia Buza1, Pei Hui

  • 1Department of Pathology, Yale University School of Medicine, New Haven, CT 06520-8023, USA. natalia.buza@yale.edu

International Journal of Gynecological Pathology : Official Journal of the International Society of Gynecological Pathologists
|March 23, 2013
PubMed
Summary

Histologic diagnosis of partial hydatidiform mole (PHM) is challenging. DNA genotyping is crucial, but specific features like cisterns and villus size ≥2.5 mm aid in diagnosis.

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Area of Science:

  • Gynecologic Pathology
  • Reproductive Genetics

Background:

  • Histologic diagnosis of partial hydatidiform mole (PHM) remains challenging.
  • DNA genotyping offers precise differentiation of PHM from mimics.
  • Overlaps exist between PHM, hydropic abortions, and chromosomal trisomies.

Purpose of the Study:

  • To reevaluate histologic parameters of PHM in correlation with DNA genotyping.
  • To identify morphologic features significant for PHM diagnosis.
  • To determine which features warrant DNA genotyping.

Main Methods:

  • 143 early abortion specimens underwent DNA genotyping.
  • Genotyping identified 60 PHM, 52 trisomies, and 31 nonmolar gestations.
  • Histologic slides were reviewed retrospectively by blinded pathologists.

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Last Updated: May 13, 2026

Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues
11:54

Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues

Published on: October 20, 2019

Measuring Single-Cell Mitochondrial DNA Copy Number and Heteroplasmy Using Digital Droplet Polymerase Chain Reaction
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Measuring Single-Cell Mitochondrial DNA Copy Number and Heteroplasmy Using Digital Droplet Polymerase Chain Reaction

Published on: July 12, 2022

Main Results:

  • Significant histologic overlaps were observed among different conditions.
  • Key diagnostic parameters for PHM included villus size, 2 villous populations, pseudoinclusions, hydrops, cisterns, and trophoblastic hyperplasia.
  • Villous hydrops (86% sensitivity) or 2 villous populations/pseudoinclusions/cisterns (84% sensitivity) were most sensitive.
  • Cisterns and villus size ≥2.5 mm showed the highest positive predictive value (90%).

Conclusions:

  • No single or combined morphologic features definitively diagnose PHM.
  • Histologic findings of round/oval pseudoinclusions, cistern formation, 2 villous populations, or villus size ≥2.5 mm should prompt DNA genotyping.