Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Water soluble fuel compounds impair metabolism in benthic foraminifera: Ecotoxicological observations at cellular level.

The Science of the total environment·2026
Same author

Minimally Destructive Radiocarbon Dating of Bone.

Rapid communications in mass spectrometry : RCM·2026
Same author

The short isoform of Tango1 is dispensable for zebrafish survival but is required for skeletal patterning and integrity.

Biology open·2025
Same author

frizzled 5 mutant zebrafish are genetically sensitised to developing microphthalmia and coloboma.

Disease models & mechanisms·2025
Same author

Autophagy regulates endosymbiont distribution in early <i>Drosophila</i> embryogenesis.

Autophagy reports·2025
Same author

Biosorption of heavy metals by microalgae: Hazardous side effects for marine organisms.

Chemosphere·2025

Related Experiment Video

Updated: May 13, 2026

Preparation of Developing and Adult Drosophila Brains and Retinae for Live Imaging
16:47

Preparation of Developing and Adult Drosophila Brains and Retinae for Live Imaging

Published on: March 15, 2010

Early stages of retinal development depend on Sec13 function.

Katy Schmidt1, Florencia Cavodeassi, Yi Feng

  • 1Cell Biology Laboratories, School of Biochemistry, Medical Sciences Building, University of Bristol, University Walk , Bristol BS8 1TD , UK ; Present address: Max F. Perutz Laboratories, University of Vienna and Medical University of Vienna, Dr-Bohr-Gasse 9/3, 1030 Wien, Austria.

Biology Open
|March 23, 2013
PubMed
Summary

The outer COPII coat protein Sec13 is crucial for transporting large amounts of cargo, like rhodopsin, essential for eye development and photoreceptor survival in zebrafish.

Keywords:
COPIIER exportEyeRhodopsinZebrafish

More Related Videos

Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster
10:10

Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster

Published on: September 20, 2014

Split Retina as an Improved Flatmount Preparation for Studying Inner Nuclear Layer Neurons in Vertebrate Retina
07:53

Split Retina as an Improved Flatmount Preparation for Studying Inner Nuclear Layer Neurons in Vertebrate Retina

Published on: January 16, 2024

Related Experiment Videos

Last Updated: May 13, 2026

Preparation of Developing and Adult Drosophila Brains and Retinae for Live Imaging
16:47

Preparation of Developing and Adult Drosophila Brains and Retinae for Live Imaging

Published on: March 15, 2010

Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster
10:10

Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster

Published on: September 20, 2014

Split Retina as an Improved Flatmount Preparation for Studying Inner Nuclear Layer Neurons in Vertebrate Retina
07:53

Split Retina as an Improved Flatmount Preparation for Studying Inner Nuclear Layer Neurons in Vertebrate Retina

Published on: January 16, 2024

Area of Science:

  • Cell Biology
  • Developmental Biology
  • Molecular Biology

Background:

  • ER-to-Golgi transport is vital for protein trafficking, mediated by the COPII coat.
  • The COPII coat, comprising Sec23/24 (inner) and Sec13/31 (outer) layers, is essential for vesicle formation.
  • Emerging evidence suggests cell- and tissue-specific regulation of COPII-mediated transport.

Purpose of the Study:

  • To investigate the role of Sec13 in zebrafish eye development.
  • To determine the necessity of the outer COPII coat (Sec13/31) for transporting large cargo proteins, specifically rhodopsin.

Main Methods:

  • Sec13 depletion in zebrafish embryos.
  • Observation of eye development and retinal structure.
  • Assessment of photoreceptor integrity, apoptosis, and proliferation.

Main Results:

  • Sec13 depletion caused photoreceptor degeneration and impaired eye development in zebrafish.
  • Defects in collagen trafficking from the retinal pigment epithelium were observed.
  • Reduced cell proliferation and increased apoptosis were noted in the retina.

Conclusions:

  • The outer COPII coat (Sec13/31) is essential for transporting large quantities of cargo proteins, including rhodopsin, not just large cargo.
  • Sec13 plays a critical role in maintaining retinal structure and photoreceptor function during development.