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Updated: May 13, 2026
piggyBac Transposon System Modification of Primary Human T Cells
Published on: November 5, 2012
piggyBac as a high-capacity transgenesis and gene-therapy vector in human cells and mice
Rongbo Li1, Yuan Zhuang, Min Han
1State Key Laboratory of Genetic Engineering and Institute of Developmental Biology and Molecular Medicine, Fudan-Yale Biomedical Research Center, School of Life Sciences, Fudan University, Shanghai 200433, China.
Abstract:
The stable genomic integration and expression of a large transgene is a major hurdle in gene therapy. We show that the modified piggyBac (PB) transposon system can be used to introduce a 207 kb genomic DNA fragment containing the RORγ/γt locus into human cells and mice. PB-mediated transgenesis results in a single copy of a stably inherited and expressed transgene. These results indicate that PB could serve as an effective high-capacity vector for functional analysis of the mammalian genome and for gene therapy in human cells.
