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Experimental mixed infection with two tick-borne viruses and interferon-mediated interference
Acta Virologica
|January 1, 1975
Summary
Tick-borne encephalitis virus (TEV) and Lipovník virus (LV) interfere with each other in cell cultures and mice. TEV infection can inhibit LV reproduction, while LV can stimulate interferon production, even when replication is blocked.
Area of Science:
- Virology
- Cell Biology
- Immunology
Background:
- Tick-borne encephalitis virus (TEV) is a Flavivirus.
- Lipovník virus (LV) is a member of the Kemerovo group (Rioviridae).
- Viral interference impacts pathogen replication and host response.
Purpose of the Study:
- To investigate the interference between TEV and LV in cell cultures and animal models.
- To characterize the effects of superinfection on viral replication and interferon production.
- To determine the co-localization of viral antigens within infected cells and tissues.
Main Methods:
- Infection of chick embryo cell (CEC) cultures with TEV and LV.
- Superinfection experiments in young and aged CEC cultures.
- Intracerebral inoculation of adult and suckling mice with TEV and LV.
- Fluorescent antibody (FA) staining for viral antigen detection.
Main Results:
- TEV preinfection inhibited LV reproduction in aged CEC cultures.
- LV superinfection stimulated interferon production in aged CEC cultures, irrespective of LV replication.
- Interference between TEV and LV was observed in both cell cultures and mouse brains.
- TEV superinfection increased the pathogenicity of LV in mice.
- Both TEV and LV antigens were found in the same cells and brain tissues.
Conclusions:
- TEV and LV exhibit significant interference in biological systems.
- Interference mechanisms involve modulation of viral replication and host interferon response.
- Co-infection is possible, with both viruses localizing to the same cells/tissues.
- Understanding viral interference is crucial for predicting disease outcomes.