Telomere lengths in human pronuclei, oocytes and spermatozoa

Telomeres are chromosome ends that control functions related to cell division. Short telomeres are proposed to underlie infertility, female reproductive ageing and abnormal embryogenesis, but there is little direct evidence on telomere length in gametes and embryos. The aim of this study was to measure telomere lengths in individual human oocytes, spermatozoa, male and female pronuclei, in order to compare parental contributions to telomere lengths in the human zygote. Quantitative fluorescence in situ hybridization was used to measure average telomere length in pronuclei of oocytes fertilized for research using a known fertile sperm sample. Pronuclei derived from male and female gametes were distinguished by 5-methylcytosine staining. Results were compared with those for unfertilized mature and immature oocytes and individual spermatozoa decondensed in vitro. Fifty unselected men and one sperm donor provided semen samples and 32 women donated oocytes surplus to IVF treatment. Telomeres in mature oocytes and female pronuclei were significantly longer than those in individual spermatozoa and male pronuclei (P < 0.0001). Telomeres were longer in immature oocytes than in mature oocytes (P < 0.04). Sperm telomere length increased with male age (P < 0.05). Neither sperm nor oocyte telomere lengths were significantly associated with clinical parameters or outcome of treatment. In conclusion, telomere length measurements directly comparing human pronuclei under identical conditions show that male-derived telomeres are shorter on average than female-derived telomeres at fertilization. We propose that from this starting point, telomere lengths are probably modified by recombination events in the oocyte until telomerase increases at the blastocyst stage. Our findings do not support the use of gamete telomere lengths as a fertility diagnostic tool.