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Updated: May 13, 2026

Cell Co-culture Patterning Using Aqueous Two-phase Systems

Cell Co-culture Patterning Using Aqueous Two-phase Systems

Published on: March 26, 2013

Polymeric Aqueous Biphasic System Rehydration Facilitates High Throughput Cell Exclusion Patterning For Cell

Hossein Tavana¹, Kerim Kaylan, Tommaso Bersano-Begey

¹Department of Biomedical Engineering, University of Akron, Akron, OH 44325 ; Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109.

Advanced Functional Materials

|March 23, 2013

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This study introduces a novel cell-exclusion patterning method using a polymeric aqueous two-phase system. This technique creates cell-free gaps for studying cell migration and enables high-throughput screening of migration inhibitors.

Area of Science:

Biotechnology
Cell Biology
Materials Science

Background:

Cell migration is crucial for biological processes like development and wound healing.
Accurate quantification of cell migration is essential for understanding disease mechanisms and drug discovery.
Existing cell migration assays can be complex and lack throughput.

Purpose of the Study:

To develop a straightforward and high-throughput method for cell-exclusion patterning.
To create a reliable assay for quantifying cell migration and detecting migration inhibitors.
To establish a cell migration niche using a polymeric aqueous two-phase system.

Main Methods:

Utilized a polymeric aqueous two-phase system (polyethylene glycol and dextran) for cell-exclusion patterning.

Keywords:

Polymeric aqueous two-phase system cell migration cell-exclusion patterning interfacial tension

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Last Updated: May 13, 2026

Cell Co-culture Patterning Using Aqueous Two-phase Systems

Cell Co-culture Patterning Using Aqueous Two-phase Systems

Published on: March 26, 2013

Robotic Production of Cancer Cell Spheroids with an Aqueous Two-phase System for Drug Testing

Robotic Production of Cancer Cell Spheroids with an Aqueous Two-phase System for Drug Testing

Published on: April 23, 2015

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Preparation of Neuronal Co-cultures with Single Cell Precision

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Formed immiscible dextran droplets on a substrate to create cell-free circular gaps.

Developed a 'gap healing' assay to visualize and quantify cell migration into these gaps.

Adapted the assay for a 96-well plate format for high-throughput screening.

Main Results:

Successfully patterned cell monolayers with well-defined circular cell-excluded gaps.
Demonstrated visualization and quantification of cell migration into the created gaps.
Showcased the assay's ability to detect inhibition of cell migration by cytoskeleton-targeting agents.
Validated the 96-well plate format for high-throughput cell migration analysis.

Conclusions:

The developed method provides a simple and effective way to create cell-exclusion patterns.
This technique facilitates robust cell migration assays with high throughput capabilities.
The method offers new opportunities for drug screening and studying cell migration dynamics.