Related Experiment Video

Updated: May 13, 2026

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae
09:15

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae

Published on: January 10, 2018

Crystallization and preliminary X-ray crystallographic studies of DnaJ from Streptococcus pneumoniae

Shasha Zhao1, Li Jin, Siqiang Niu

  • 1Key Laboratory of Molecular Biology on Infectious Disease, Chongqing Medical University, YiXueYuanlu-1, Chongqing 400016, People's Republic of China.

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
|March 23, 2013
PubMed

Insights

This study reports the expression, purification, and preliminary crystallographic analysis of Streptococcus pneumoniae DnaJ (SpDnaJ), a protein involved in bacterial infection and vaccine development.

Area of Science:

  • Molecular Biology
  • Structural Biology
  • Biochemistry

Background:

  • DnaJ proteins are molecular chaperones essential for protein folding, complex dissociation, and membrane translocation.
  • DnaJ from Streptococcus pneumoniae (SpDnaJ) plays a role in bacterial infections and is a target for vaccine development.

Purpose of the Study:

  • To report the expression, purification, crystallization, and preliminary crystallographic analysis of SpDnaJ.
  • To provide structural insights into SpDnaJ for potential therapeutic applications.

Main Methods:

  • Bacterial expression and purification of SpDnaJ.
  • Crystallization of SpDnaJ.
  • Preliminary X-ray diffraction analysis.

Main Results:

  • SpDnaJ was successfully expressed and purified.
  • Crystals of SpDnaJ were obtained, belonging to space groups I222 or I2₁2₁2₁.
  • Diffraction data were collected to a resolution of 3.0 Å, with unit-cell parameters a=47.68, b=104.45, c=234.57 Å.

Conclusions:

  • The preliminary crystallographic data provide a foundation for future structure determination of SpDnaJ.
  • Understanding the structure of SpDnaJ can aid in the development of novel vaccines against Streptococcus pneumoniae infections.
Keywords:
DnaJStreptococcus pneumoniae

More Related Videos

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering07:19

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Published on: November 5, 2018

Neutron Crystallography Data Collection and Processing for Modelling Hydrogen Atoms in Protein Structures10:10

Neutron Crystallography Data Collection and Processing for Modelling Hydrogen Atoms in Protein Structures

Published on: December 1, 2020

Related Concept Videos

X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal crystal...
X-ray Crystallography02:18

X-ray Crystallography

The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
Determination of Crystal Structures01:29

Determination of Crystal Structures

In the late 1800s, the revelation that light extended beyond visible wavelengths led to the discovery of X-rays by Wilhelm Roentgen. Recognized as high-energy electromagnetic radiation with short wavelengths, X-rays prompted exploration into their interaction with crystals. Max von Laue proposed in 1912 that the periodic arrangement of atoms, ions, or molecules in crystals would cause them to diffract X-rays, a hypothesis confirmed through experiments with copper sulfate and zinc sulfide...