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[The new technologies for the analytical examination of the embryonic metabolome and its prospects]
1Gynekologicko-porodnicka klinika LF MU a FN Brno. icrha@fnbrno.cz
Objective:
To review current technologies for the analytical examination of the embryonic metabolome and its perspectives.
Design:
Review article.
Setting:
Department of Gynecology and Obstetrics, Faculty of Medicine, Masaryk University, and University Hospital, Brno, Department of Biochemistry, Faculty of Science and CEITEC, Masaryk University, Brno.
Methods And Results:
Nowadays, very sensitive analytical technologies are available. They enable exact measurement of various molecules - products of embryo metabolism during first days of cultivation. The capillary electrophoresis is one of promising method. Recent studies analysed metabolic differences between embryos that result in a pregnancy and those that do not. Amino acid levels, glucose or pyruvate in the embryo culture media were analysed most frequently. However, results of these studies are ambiguous.
Conclusions:
The capillary electrophoresis with contactless conductivity detection may provide a useful data of the embryonic metabolome. A comprehensive analysis of the used culture medium may represent a valuable adjunct to morphological criteria for enhanced rates of implantation and delivery.
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