Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Metabolome-wide association identifies altered metabolites and metabolic pathways in the serum of patients with cholangiocarcinoma.

JHEP reports : innovation in hepatology·2024
Same author

Dysregulated Cerebrospinal Fluid Proteome of Spinocerebellar Ataxia Type 2 and its Clinical Implications.

Movement disorders : official journal of the Movement Disorder Society·2024
Same author

Defining albumin as a glycoprotein with multiple N-linked glycosylation sites.

Journal of translational medicine·2024
Same author

D-mannose as a new therapy for fucokinase deficiency-related congenital disorder of glycosylation (FCSK-CDG).

Molecular genetics and metabolism·2024
Same author

Liposome-encapsulated mannose-1-phosphate therapy improves global N-glycosylation in different congenital disorders of glycosylation.

Molecular genetics and metabolism·2024
Same author

A green and economic approach to synthesize magnetic Lagenaria siceraria biochar (γ-Fe<sub>2</sub>O<sub>3</sub>-LSB) for methylene blue removal from aqueous solution.

Environmental science and pollution research international·2024

Related Experiment Video

Updated: May 13, 2026

Quantitation of Rabies Virus in Various Bovine Brain Structures
13:42

Quantitation of Rabies Virus in Various Bovine Brain Structures

Published on: May 22, 2021

Quantitative proteomics for identifying biomarkers for Rabies.

Abhilash K Venugopal1, S Sameer Kumar Ghantasala, Lakshmi Dhevi N Selvan

  • 1McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University, 733 N, Broadway, BRB 527, Baltimore, MD, 21205, USA. pandey@jhmi.edu.

Clinical Proteomics
|March 26, 2013
PubMed
Summary

This study used quantitative proteomics to identify novel protein biomarkers for distinguishing between encephalitic and paralytic rabies. Findings highlight potential diagnostic markers in cerebrospinal fluid for early rabies detection.

More Related Videos

Whole Genome Sequencing for Rapid Characterization of Rabies Virus Using Nanopore Technology
10:26

Whole Genome Sequencing for Rapid Characterization of Rabies Virus Using Nanopore Technology

Published on: August 18, 2023

Postmortem Diagnosis of Rabies in Animals by the Updated, Multiplexed LN34 Real-Time Reverse Transcription-Polymerase Chain Reaction Assay
09:54

Postmortem Diagnosis of Rabies in Animals by the Updated, Multiplexed LN34 Real-Time Reverse Transcription-Polymerase Chain Reaction Assay

Published on: May 2, 2025

Related Experiment Videos

Last Updated: May 13, 2026

Quantitation of Rabies Virus in Various Bovine Brain Structures
13:42

Quantitation of Rabies Virus in Various Bovine Brain Structures

Published on: May 22, 2021

Whole Genome Sequencing for Rapid Characterization of Rabies Virus Using Nanopore Technology
10:26

Whole Genome Sequencing for Rapid Characterization of Rabies Virus Using Nanopore Technology

Published on: August 18, 2023

Postmortem Diagnosis of Rabies in Animals by the Updated, Multiplexed LN34 Real-Time Reverse Transcription-Polymerase Chain Reaction Assay
09:54

Postmortem Diagnosis of Rabies in Animals by the Updated, Multiplexed LN34 Real-Time Reverse Transcription-Polymerase Chain Reaction Assay

Published on: May 2, 2025

Area of Science:

  • Neuroscience
  • Proteomics
  • Infectious Diseases

Background:

  • Rabies is a fatal viral disease affecting the central nervous system, posing a significant public health threat in Asia and Africa.
  • Clinical rabies presents as encephalitic (furious) or paralytic (numb) forms.
  • Prompt diagnosis is critical for rabies prevention through post-exposure prophylaxis, as the disease is invariably fatal if untreated.

Purpose of the Study:

  • To conduct a quantitative proteomic analysis of human brain tissue to identify differentially expressed proteins between encephalitic and paralytic rabies subtypes.
  • To discover novel protein biomarkers for improved antemortem diagnosis of rabies.

Main Methods:

  • Quantitative proteomic analysis using an 8-plex isobaric tags for relative and absolute quantification (iTRAQ) strategy.
  • Comparison of protein expression profiles in human brain tissue from encephalitic rabies, paralytic rabies, and normal controls.

Main Results:

  • Identified 402 proteins, with several showing differential expression between rabies subtypes.
  • Karyopherin alpha 4 (KPNA4) overexpressed in paralytic rabies; Calcium calmodulin dependent kinase 2 alpha (CAMK2A) upregulated in paralytic rabies.
  • Glutamate ammonia ligase (GLUL) overexpressed in both paralytic and encephalitic rabies.

Conclusions:

  • GLUL and CAMK2A were validated as upregulated molecules in rabies.
  • Further investigation of these proteins in cerebrospinal fluid is recommended for potential use as antemortem diagnostic biomarkers.
  • This study represents the first systematic proteomic profiling of human rabies clinical subtypes.