Simultaneous detection of lactate and glucose by integrated printed circuit board based array sensing chip

Area of Science:

• Biomedical Engineering
• Biosensor Technology
• Nanomaterials

Background:

• Developing sensitive and specific biosensors for simultaneous detection of biomarkers like lactate and glucose is crucial for diagnostics.
• Existing methods often face challenges with cost, throughput, and specificity.

Purpose of the Study:

• To develop an integrated printed circuit board (PCB) based array sensing chip for simultaneous lactate and glucose detection.
• To demonstrate an inexpensive, high-throughput electronic biochip with high signal-to-noise ratio and sensitivity.

Main Methods:

• Fabrication of a composite sensing platform using chitosan, Polyvinylimidazole-Os (PVI-Os), and carbon nanotubes (CNTs).
• Utilizing electrostatic immobilization of enzymes onto the positively charged chitosan/PVI-Os mediator.
• Incorporating additional electrodes without enzymes for interference correction to enhance specificity.

Main Results:

• Achieved low detection limits of 0.6 μM for lactate and 5 μM for glucose.
• Demonstrated high sensitivity and specificity through the novel composite sensing platform.
• Validated the potential for high signal-to-noise ratio and reproducible measurements.

Conclusions:

• The developed PCB-based array sensing chip offers a promising platform for cost-effective, high-throughput, and sensitive simultaneous detection of lactate and glucose.
• The integrated design and composite sensing layer provide a foundation for extending this technology to various other sensor applications.