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Updated: May 13, 2026
Transient Transduction of the Strobilated Forms of Echinococcus granulosus
Published on: September 16, 2022
[Effect of Echinococcus granulosus cyst fluid on the proliferation of HepG2 cells]
Chao-wang Li1, Jin-ming Zhao, Chuan-shan Zhang
1Department of Breast Surgery and Pediatric Surgery, First Affiliated Hospital of Xinjiang Medical University, Urumqi, China.
Objective:
To investigate whether Echinococcus granulosus cyst fluid-infected host liver cells had differential expression of mitogen-activated protein kinases (MAPKs) or differential cell cycle activity.
Methods:
Human liver cells cultured with different concentrations of hydatid cyst fluid (HCF) were tested by the MTT method to determine effects on proliferation. The cell cycle was assessed by flow cytometry. Western blotting was used to detect changes in protein expressions of p-ERK, PCNA, cyclin-A, cyclin-B1, cyclin-D1, and cyclin-E.
Results:
Forty-eight, 72 and 96 h of HCF at 15%, 30% and 60% concentrations in the cell media significantly promoted cell proliferation (F=67.845, P less than 0.01) and compared to controls (P less than 0.05). Cells exposed to 15% HCF for 48 h showed significantly induced expression of p-ERK (F=1.916, P less than 0.01), higher than controls (P less than 0.01). Cells exposed to 15% HCF for 24 h showed significantly induced expression of cyclin-Dl (F=3.901, P less than 0.01), higher than controls (P less than 0.01). Cells exposed to 15% HCF for 48 h or 30% HCF for 72 h showed significantly induced expression of PCNA (F=91.140, P less than 0.01), higher than controls (P less than 0.01). Cells exposed to 15% HCF for 48 h or 30% HCF for 72 h shed significantly induced expression of cyclin-A (F=18.587, P=0.002), higher than controls (P less than 0.01). Cells exposed to 15% HCF for either 48 h or 72 h showed significantly induced expression of cyclin-B1 (F=2.064, P less than 0.01), higher than controls (P less than 0.01). Cells exposed to 30% HCF for 96 h showed significantly induced expression of cyclin-E (F=1.068, P less than 0.01), higher than controls (P less than 0.01).
Conclusion:
Hydatid cyst fluid exerts no inhibitory effect on primary cultured host liver cells, but may promote cellular proliferation.
Insights
Hydatid cyst fluid (HCF) from Echinococcus granulosus significantly promotes liver cell proliferation. HCF exposure upregulates key proteins involved in cell cycle progression and mitogen-activated protein kinase signaling pathways.
Area of Science:
- Parasitology
- Cell Biology
- Molecular Biology
Context:
- Echinococcus granulosus infection can lead to liver cyst formation.
- The biological effects of hydatid cyst fluid (HCF) on host cells are not fully understood.
- Investigating host-parasite interactions at the cellular level is crucial for understanding disease pathogenesis.
Purpose:
- To determine the effect of Echinococcus granulosus cyst fluid (HCF) on host liver cell proliferation.
- To investigate the differential expression of mitogen-activated protein kinases (MAPKs) and cell cycle activity in HCF-exposed liver cells.
- To analyze the expression of specific proteins including p-ERK, PCNA, cyclin-A, cyclin-B1, cyclin-D1, and cyclin-E.
Summary:
- Human liver cells treated with varying concentrations of HCF exhibited significantly enhanced proliferation.
- HCF exposure led to increased expression of p-ERK, a key MAPK pathway component.
- Upregulation of cell cycle regulatory proteins such as cyclin-D1, PCNA, cyclin-A, and cyclin-B1 was observed, indicating promotion of cell cycle progression.
- Cyclin-E expression was also significantly induced by HCF treatment.
Impact:
- Hydatid cyst fluid does not inhibit primary host liver cells but promotes their proliferation.
- Findings suggest HCF plays a role in modulating host cell behavior during Echinococcus granulosus infection.
- This research provides insights into the molecular mechanisms underlying host cell responses to parasitic cyst fluids.
