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Translation Efficiency Test Using Polysome Profiles Under Heat Stress
Published on: October 11, 2024
Genome-wide analysis and molecular characterization of heat shock transcription factor family in Glycine max
Eunsook Chung1, Kyoung-Mi Kim, Jai-Heon Lee
1BK21 Center for Silver-Bio Industrialization, College of Natural Resources and Life Science, Dong-A University, Hadan 2 dong, Sahagu, Busan 604-714, Republic of Korea.
Abstract:
Heat shock transcription factors (Hsfs) play an essential role on the increased tolerance against heat stress by regulating the expression of heat-responsive genes. In this study, a genome-wide analysis was performed to identify all of the soybean (Glycine max) GmHsf genes based on the latest soybean genome sequence. Chromosomal location, protein domain, motif organization, and phylogenetic relationships of 26 non-redundant GmHsf genes were analyzed compared with AtHsfs (Arabidopsis thaliana Hsfs). According to their structural features, the predicted members were divided into the previously defined classes A-C, as described for AtHsfs. Transcript levels and subcellular localization of five GmHsfs responsive to abiotic stresses were analyzed by real-time RT-PCR. These results provide a fundamental clue for understanding the complexity of the soybean GmHsf gene family and cloning the functional genes in future studies.
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