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Related Concept Videos

Centrioles and Centrosomes01:13

Centrioles and Centrosomes

Most animal cells comprise a pair of centrioles together called a centrosome. The cell duplicates its centrosome and contains two centrosomes side-by-side, which begin to move apart during the prophase. As the centrosomes migrate to two different sides of the cell, microtubules start extending from each centrosome toward the other end. The mitotic spindle is composed of the centrosomes and their emerging microtubules.
Near the end of the prophase, also called late prophase or "prometaphase,"...

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Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
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Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

Centrosome isolation and analysis by mass spectrometry-based proteomics.

Lis Jakobsen1, Jacob Morville Schrøder, Katja M Larsen

  • 1Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark.

Methods in Enzymology
|March 26, 2013
PubMed
Summary

Centrioles are vital microtubule scaffolds. This study details methods for isolating human cell centrosomes and analyzing associated proteins using mass spectrometry, advancing our understanding of their complex roles.

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Last Updated: May 13, 2026

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Area of Science:

  • Cell Biology
  • Proteomics
  • Molecular Biology

Background:

  • Centrioles are microtubule structures crucial for centrosome, cilia, and flagella formation.
  • These organelles play key roles in cell cycle, physiology, and development.
  • Previous studies identified conserved centriole-associated proteins, but their molecular functions remain unclear.

Purpose of the Study:

  • To develop methods for large-scale isolation of centrosomes from human cells.
  • To employ quantitative mass spectrometry for identifying and characterizing centriole-associated proteins.
  • To gain deeper insights into the molecular mechanisms of centriole-mediated processes.

Main Methods:

  • Isolation of centrosomes from human cell lines.
  • Quantitative mass spectrometry-based proteomics for protein identification.
  • Bioinformatic analysis of proteomic data to identify and study protein properties.

Main Results:

  • Successfully established protocols for purifying centrosomes from human cells.
  • Identified a comprehensive set of centriole-associated proteins.
  • Characterized the properties of these proteins, providing a foundation for further functional studies.

Conclusions:

  • The developed methods enable thorough proteomic analysis of centrosomes.
  • This work expands the catalog of known centriole-associated proteins.
  • Further investigation of these proteins will illuminate the molecular basis of cilia and centrosome functions.