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Related Experiment Video

Updated: May 13, 2026

Intraductal Injection of LPS as a Mouse Model of Mastitis: Signaling Visualized via an NF-κB Reporter Transgenic

Intraductal Injection of LPS as a Mouse Model of Mastitis: Signaling Visualized via an NF-κB Reporter Transgenic

Published on: September 4, 2012

Immunotherapy of mastitis.

Gabriel Leitner¹, Yosef Pinchasov, Eli Morag

¹National Mastitis Reference Center, Kimron Veterinary Institute, Bet Dagan, Israel.

Veterinary Immunology and Immunopathology

|March 26, 2013

Summary

A novel Y-complex treatment effectively cleared mastitis-causing bacteria in cows. This immunologically based approach proved safe and effective, offering a promising alternative to traditional antibiotic treatments for bovine mastitis.

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Isolation and Culture of Primary Human Mammary Epithelial Cells

Isolation and Culture of Primary Human Mammary Epithelial Cells

Published on: May 3, 2024

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Last Updated: May 13, 2026

Intraductal Injection of LPS as a Mouse Model of Mastitis: Signaling Visualized via an NF-κB Reporter Transgenic

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Published on: September 4, 2012

Isolation and Culture of Primary Human Mammary Epithelial Cells

Isolation and Culture of Primary Human Mammary Epithelial Cells

Published on: May 3, 2024

Area of Science:

Veterinary immunology
Bovine mastitis research
Microbial pathogenesis

Background:

Mastitis poses a significant threat to dairy cattle health and productivity.
Current treatments often involve antibiotics, raising concerns about resistance and milk withdrawal periods.
There is a need for alternative, effective mastitis therapies.

Purpose of the Study:

To evaluate an innovative, immunology-based treatment for mastitis.
To assess the efficacy and safety of Y-complex, a microbead carrying antibodies and a phagocytosis enhancer.
To compare Y-complex with conventional antibiotic treatments and a non-steroidal anti-inflammatory drug (NSAID).

Main Methods:

Two experiments were conducted, involving challenged and naturally infected cows.
Experiment 1: Cows challenged with Streptococcus dysgalactiae were treated with Y-complex, Cobactan LC®, or saline.
Experiment 2: Field study with naturally infected cows treated with Y-complex, broad-spectrum antibiotics (BA), or NSAID.

Main Results:

Y-complex rapidly cleared S. dysgalactiae in challenged cows, with sustained efficacy.
In field trials, Y-complex demonstrated comparable efficacy to antibiotics in eliminating bacteria.
Y-complex treatment resulted in less discarded milk compared to antibiotic treatments, with similar milk quality and quantity outcomes.

Conclusions:

Y-complex is a safe and effective immunologically based treatment for bovine mastitis.
This novel approach offers a viable alternative to antibiotic therapy.
Y-complex shows potential to reduce economic losses associated with mastitis treatment.