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Interactions of CT-DNA with Hypocrellin A and its Al(3+)-Hypocrellin A complex
Fei Ma1, Xuefeng Ge, Heyong Huang
1Analysis and Testing Center, School of Geograph Science, Jiangsu Key Laboratory of Biofunctional Materials, Nanjing Normal University, Nanjing 210046, PR China.
Abstract:
In this study, the chelation of Hypocrellin A (HA) with Al(3+) in water solution has been synthesized, and the interactions of HA and Al(3+)-HA complex with calf thymus DNA are in detail compared by UV-vis and fluorescence spectroscopic techniques, circular dichroism spectroscopy and viscosity measurement. The experiment results suggest that HA and Al(3+)-HA complex both could bind to CT DNA by intercalation mode, and double helix of DNA was damaged. Moreover, Al(3+)-HA complex not only displays higher absorption at therapeutic window but also displays stronger binding affinity to CT DNA than HA.
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