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Updated: May 13, 2026

Expression of Fluorescent Proteins in Branchiostoma lanceolatum by mRNA Injection into Unfertilized Oocytes
Published on: January 12, 2015
A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum
Nathan C Shaner1, Gerard G Lambert, Andrew Chammas
1Department of Photobiology and Bioimaging, The Scintillon Institute, San Diego, California, USA. nathanshaner@scintillon.org
Abstract:
We report a monomeric yellow-green fluorescent protein, mNeonGreen, derived from a tetrameric fluorescent protein from the cephalochordate Branchiostoma lanceolatum. mNeonGreen is the brightest monomeric green or yellow fluorescent protein yet described to our knowledge, performs exceptionally well as a fusion tag for traditional imaging as well as stochastic single-molecule superresolution imaging and is an excellent fluorescence resonance energy transfer (FRET) acceptor for the newest cyan fluorescent proteins.
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