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Updated: May 13, 2026

A Microfluidic Chip for ICPMS Sample Introduction

A Microfluidic Chip for ICPMS Sample Introduction

Published on: March 5, 2015

A multifunctional microfluidic droplet-array chip for analysis by electrospray ionization mass spectrometry.

Yuan Su¹, Ying Zhu, Qun Fang

¹Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou, China.

|March 26, 2013

Summary

This study introduces a novel droplet-array chip system for streamlined sample preparation and analysis using electrospray ionization mass spectrometry (ESI-MS). The integrated chip and L-shaped capillary interface enable efficient, automated multi-step pretreatment and identification of proteomic samples.

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Last Updated: May 13, 2026

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A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

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Published on: October 15, 2013

Area of Science:

Analytical Chemistry
Mass Spectrometry
Microfluidics

Background:

Traditional sample preparation for mass spectrometry is often time-consuming and requires significant sample volumes.
Integrating sample pretreatment with direct mass spectrometry analysis can improve efficiency and reduce sample loss.
Droplet-based microfluidic systems offer advantages in handling small sample volumes and performing complex reactions.

Purpose of the Study:

To develop and validate a multifunctional semi-closed droplet-array chip coupled with electrospray ionization mass spectrometry (ESI-MS).
To establish a novel interfacing strategy for seamless integration of droplet arrays with ESI-MS.
To demonstrate the system's capability for multi-step pretreatment and analysis of proteomic samples.

Main Methods:

A 2D droplet-array chip (8x8 microwell array) covered with oil was used for sample storage and protection.
An L-shaped capillary served as both a sampling probe and an electrospray emitter, directly interfacing with the droplet array.
The system was applied to perform in-droplet protein reduction, alkylation, digestion, and solid-phase extraction (SPE) prior to ESI-MS analysis.

Main Results:

The system achieved efficient, automated multi-step pretreatment and identification of myoglobin and cytochrome C.
Analysis time per droplet was 40 seconds with a low sample consumption of approximately 13 nL.
Good repeatability (5.7% RSD) was demonstrated for reserpine droplet analysis, highlighting system reliability.

Conclusions:

The developed semi-closed droplet-array chip coupled with ESI-MS offers a flexible and controllable platform for automated sample analysis.
The novel interfacing method eliminates the need for separate droplet extraction devices, simplifying the workflow.
This integrated system shows significant potential for high-throughput analysis of small-volume proteomic and other biological samples.