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Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
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Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

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Related Experiment Video

Updated: May 13, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Chip-based nLC-TOF-MS is a highly stable technology for large-scale high-throughput analyses.

L Renee Ruhaak1, Sandra L Taylor, Suzanne Miyamoto

  • 1Department of Chemistry, University of California Davis, Davis, CA 95616, USA. lruhaak@ucdavis.edu

Analytical and Bioanalytical Chemistry
|March 26, 2013
PubMed
Summary

Chip-based nano-liquid chromatography (LC)-mass spectrometry (MS) offers high stability and repeatability for biomarker discovery. This technology is suitable for analyzing large numbers of clinical samples, improving diagnostic and treatment strategies.

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Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)
07:34

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)

Published on: March 14, 2013

Related Experiment Videos

Last Updated: May 13, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)
07:34

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)

Published on: March 14, 2013

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Biotechnology

Background:

  • Mass spectrometry (MS) facilitates biomarker discovery for disease diagnosis and treatment.
  • High-performance liquid chromatography (HPLC) coupled to MS is widely used.
  • Miniaturization via nano-LC-MS enhances sensitivity but often limits repeatability.

Purpose of the Study:

  • To evaluate the temporal repeatability of chip-based nano-LC-MS.
  • To assess the stability of chip-based nano-LC-MS for biomarker discovery applications.

Main Methods:

  • N-glycans were released from a serum sample.
  • Repeated analyses were performed using nano-LC-Proteomics-chip-Time-of-Flight-MS (nLC-PGC-chip-TOF-MS) over three non-consecutive days.
  • Inter-day coefficient of variation was calculated on log10-transformed integrals.

Main Results:

  • Chip-based nano-LC-MS demonstrated very high temporal repeatability.
  • The average inter-day coefficient of variation was 4%.

Conclusions:

  • Chip-based nano-LC-MS is a highly stable technology.
  • This technique is suitable for profiling large cohorts of clinical samples for biomarker discovery.
  • Improved stability enhances the utility of nano-LC-MS in clinical research.