Related Experiment Video
Updated: May 13, 2026

Isolation of Human Monocytes by Double Gradient Centrifugation and Their Differentiation to Macrophages in Teflon-coated Cell Culture Bags
Published on: September 9, 2014
Regulation of monocyte differentiation by specific signaling modules and associated transcription factor networks
René Huber1, Daniel Pietsch, Johannes Günther
1Institute of Clinical Chemistry, Hannover Medical School, Carl-Neuberg-Str.1, 30625, Hannover, Germany, huber.rene@mh-hannover.de.
Abstract:
Monocyte/macrophages are important players in orchestrating the immune response as well as connecting innate and adaptive immunity. Myelopoiesis and monopoiesis are characterized by the interplay between expansion of stem/progenitor cells and progression towards further developed (myelo)monocytic phenotypes. In response to a variety of differentiation-inducing stimuli, various prominent signaling pathways are activated. Subsequently, specific transcription factors are induced, regulating cell proliferation and maturation. This review article focuses on the integration of signaling modules and transcriptional networks involved in the determination of monocytic differentiation.
Related Concept Videos
Differentiation of Common Myeloid Progenitor Cells
Master Transcription Regulators
Lineage Commitment
Regulation of Hematopoietic Stem Cells

