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Updated: May 13, 2026

08:08
Isolation and Activation of Murine Lymphocytes
Published on: October 30, 2016
Quantifying CFSE Label Decay in Flow Cytometry Data
1Center for Research in Scientific Computation, Center for Quantitative Sciences in Biomedicine, North Carolina State University, Raleigh, NC.
Abstract:
We developed a series of models for the label decay in cell proliferation assays when the intracellular dye carboxyfluorescein succinimidyl ester (CFSE) is used as a staining agent. Data collected from two healthy patients were used to validate the models and to compare the models with the Akiake Information Criteria. The distinguishing features of multiple decay rates in the data are readily characterized and explained via time dependent decay models such as the logistic and Gompertz models.

