Bioanalytical method development, validation and quantification of dorsomorphin in rat plasma by LC-MS/MS

Area of Science:

• Pharmacology
• Analytical Chemistry
• Biotechnology

Background:

• Dorsomorphin is a compound with potential therapeutic applications.
• Accurate quantification of dorsomorphin in biological matrices is crucial for pharmacokinetic studies.
• Existing methods may lack the required sensitivity or specificity for rat plasma analysis.

Purpose of the Study:

• To develop and validate a sensitive liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) method for the quantification of dorsomorphin in rat plasma.
• To establish a reliable analytical tool for pharmacokinetic profiling of dorsomorphin.

Main Methods:

• Development of a sensitive LC-MS/MS method utilizing multiple reaction monitoring (MRM) mode.
• Chromatographic separation on an Agilent XDB C18 column with a mobile phase of acetonitrile and ammonium acetate buffer.
• Zaleplon used as an internal standard for accurate quantification.
• Positive ion mode electrospray ionization (ESI) for detection.

Main Results:

• The developed LC-MS/MS method demonstrated high sensitivity and specificity for dorsomorphin in rat plasma.
• Linearity was established over a concentration range of 0.005 to 10 µg/mL with a correlation coefficient (r²) ≥ 0.99.
• Mean extraction recovery for dorsomorphin and the internal standard exceeded 80%.
• Inter-day and intra-day precision and accuracy were within acceptable limits (85-115% CV).

Conclusions:

• A robust and validated LC-MS/MS method for dorsomorphin estimation in rat plasma has been successfully developed.
• This method is suitable for determining the oral pharmacokinetic profile of dorsomorphin in rats.
• The validated assay provides a valuable tool for preclinical research involving dorsomorphin.